Search by BoMiProt ID - Bomi6828


Primary Information

BoMiProt ID Bomi6828
Protein Name Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial/Branched-chain alpha-keto acid dehydrogenase complex component E2/ Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase/Dihydrolipoamide branched chain transacylase/Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex/BCKAD-E2/BCKADE2
Organism Bos taurus
Uniprot IDP11181
Milk FractionWhey
Ref Sequence ID NP_776330.1
Aminoacid Length 482
Molecular Weight 53410
FASTA Sequence Download
Gene Name DBT
Protein Existence Status Reviewed

Secondary Information

Protein Function Lipoamide acyltransferase component (E2)of The branched-chain alpha-keto dehydrogenase complex. Its function is to transfer of the acyl group from the lipoyl moiety to coenzyme A.the function of The branched-chain alpha-keto dehydrogenase complex is conversion of alpha-keto acids to acyl-CoA and CO2.
Biochemical Properties The 52 000-dalton subunit is the E2 transacylase that contains the functional lipoyl moiety.
PTMs Acetylation on Lys and Phosphorylation on Ser
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|P11181|ODB2_BOVIN Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Bos taurus OX=9913 GN=DBT PE=1 SV=2 MAAALVLRTWSRAAGQLICVRYFQTCGNVHVLKPKYVCFFGYPPFKYSHPYQWLKTTAAL QGQIVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKK LYYNLDDTAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGQKTLATPAVRRL AMENNIKLSEVIGSGKDGRILKEDILNYLEKQTGAILPPS*120PKAEIMPPPPKPKDRTIPIP ISKPPVFIGKDRTEPVKGFHKAMVKTMSAALKIPHFGYCDEVDLTELVKLREELKPIAFA RGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVK NVQIRSIFEIATELNRLQKLGSAGQLSTNDLIGGTFTLSNIGSIGGTYAKPVILPPEVAI GALGTIKALPRFNEKGEVCKAQIMNVSWSADHRIIDGATVSRFSNLWKSYLENPAFMLLD LK
SCOP Class : Alpha and beta proteins (a/b)
Fold : CoA-dependent acyltransferases
Superfamily : CoA-dependent acyltransferases
Family : CAT-like
Domain Name : 2IHW A:188-421

CATH Matched CATH superfamily
3.30.559.10
Predicted Disorder Regions 142-171, 213-244
DisProt Annotation
TM Helix Prediction No TM helices
PDB ID 2IHW,2II3,2II4,2II5,
Bibliography 1.Heffelfinger SC, Sewell ET, Danner DJ. Identification of specific subunits of highly purified bovine liver branched-chain ketoacid dehydrogenase. Biochemistry. 1983 Nov 22;22(24):5519-22. doi: 10.1021/bi00293a011. PMID: 6652074. 2.Pettit FH, Yeaman SJ, Reed LJ. Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4881-5. doi: 10.1073/pnas.75.10.4881. PMID: 283398; PMCID: PMC336225.