Primary Information |
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| BoMiProt ID | Bomi6495 |
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| Protein Name | Interphotoreceptor matrix proteoglycan 1/Mucin-like glycoprotein associated with photoreceptor cells/Sialoprotein associated with cones and rods |
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| Organism | Bos taurus |
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| Uniprot ID | Q9GMS5 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_776787.1 |
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| Aminoacid Length | 794 |
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| Molecular Weight | 89378 |
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| FASTA Sequence |
Download |
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| Gene Name | IMPG1/MLGAPC |
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| Gene ID | 281866 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | sialoprotein,expressed in both rod and cone photoreceptor cells. SPACR and hyaluronan may serve to form the basic macromolecular scaffold, which comprises the insoluble interphotoreceptor matrix. |
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| Biochemical Properties | have hyaluronan (HA) binding motifs of the receptor for HA-mediated motility (RHAMM) type. EGF-like domains require calcium for their biological function.EGF-like domain in IMPG1/SPACR contains the critical asparagine (Asn702), which has been implicated in calcium binding. |
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| PTMs | Highly glycosylated (N- and O-linked carbohydrates and sialic acid).Disulfide bond formation. |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q9GMS5|IMPG1_BOVIN Interphotoreceptor matrix proteoglycan 1 OS=Bos taurus OX=9913 GN=IMPG1 PE=2 SV=1
MHLEAARVIFFLWIFLQVQGIKDLSIKIYGSEIKDIDNAPRTEATKNTAKTYKVSTMRRI
FDLAKHRTKRSAFFPTGVKVCPQESMEQILASLQAYYRLRVCQEAVWEAYRIFLDRLPEP
GEYQDWVSVCQQETFCLFDIGKNFSNSQEHLDLLQQRMKQRNFLERKDEVVTKETLGELG
QTPGLQQTLPVSHPGPCLSLPMTTAQRNPQLHPSRTPRVPTRERKIEFTDAAEDALEQKV
ELSISLANQKFKSELDNSQSPYYLEVAAKSQLQMQKIFKKLPGFKEIHVSGFRPKKERDG
TSSTEMQLTAIFKKGKAEAKSPASDPLSLDSNKIESEGDPRGTTEEEKQRELYPTASELR
KLISRALEEDQSLDVGTIQFTDEIVGSLPSLDPDTQLVLPTLLTDITKDATLSPELPLGQ
PRLET*425VDRAGHSPPGASPT*439DGWS*443PPAMT*448ST*450SLSETLPFFTASSVFPQTDQSATDIMSIDQTVLIPRLTVPTDDYSAISPLVPEISHLPTSSEDWLSTSSQDTMEYLDGVDLTKTPTSSEG
PRNSVGMFPAWIIFLENITPDPGLRYITTSAMTVAARGRELVVFFSLRVANVPFSTDLFN
KSSLEYQALEQRFTQLLVPNLRSN*624LTGFKQLEILNFRNGSVIVNSKVRFAKSVPYN*656LTKA
VRGVLEDFRSTAAQQLDLEIDSYSLDVEPADQADPCKFLACGEFAQCVRNEWTEEAECRC
RSGTQALVLPIEDCEDIPGKGTPCRSLDQSKNQVYEPGVKKFQRQQDNKVTMKRKFELLT
IGYEEFNYQDWEGN
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| Predicted Disorder Regions | 166-223, 298-302, 316-325, 344-473, 503-542 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | Seven potential N-linked glycosylation sites are present in two clusters on either side of a mucin-like domain containing potentialO-linked glycosylation sites (from residue Gly296 to Pro552).The six conserved cysteine residues present in EGF like domain are involved in intrachain disulfide bonding that is required for the structural stability . |
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| Bibliography | 1.Acharya S, Rodriguez IR, Moreira EF, Midura RJ, Misono K, Todres E, Hollyfield JG. SPACR, a novel interphotoreceptor matrix glycoprotein in human retina that interacts with hyaluronan. J Biol Chem. 1998 Nov 20;273(47):31599-606. doi: 10.1074/jbc.273.47.31599. PMID: 9813076. 2.Brandl C, Schulz HL, Charbel Issa P, Birtel J, Bergholz R, Lange C, Dahlke C, Zobor D, Weber BHF, Stöhr H. Mutations in the Genes for Interphotoreceptor Matrix Proteoglycans, IMPG1 and IMPG2, in Patients with Vitelliform Macular Lesions. Genes (Basel). 2017 Jun 23;8(7):170. doi: 10.3390/genes8070170. PMID: 28644393; PMCID: PMC5541303. |
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