Search by BoMiProt ID - Bomi6416


Primary Information

BoMiProt ID Bomi6416
Protein Name Insulin-like growth factor-binding protein 6/IBP-6/IGF-binding protein 6/IGFBP-6
Organism Bos taurus
Uniprot IdQ05718
Milk FractionWhey
Ref Sequence Id NP_001035585.1
Aminoacid Length 237
Molecular Weight 24967
Fasta Sequence https://www.uniprot.org/uniprot/Q05718.fasta
Gene Name IGFBP6
Gene Id 404186
Protein Existence Status reviewed

Secondary Information

Protein Function inhibition of angiogenesis and promotion of cancer cell migration
Biochemical Properties share a three-domain structure.The N-domain contains a highly conserved high-affinity IGF binding subdomain that has two disulphide linkages stabilising a globular structure containing a three-stranded anti-parallel β-sheet.The N-terminal subdomains of IGFBPs 1–5 contain a conserved GCGCC motif and a ladder-like structure stabilised by four disulphide bonds.IGFBP-6 differs from other IGFBPs as it lacks this cysteine-rich motif and only has three disulphide bonds. Further, a peptide based on this subdomain is predominantly extended with minimal secondary structure.The C-domain contains three homologous disulphide linkages, a conserved CWCV sequence and a thyroglobulin type 1 fold comprising an α -helix followed by a loop, a three-stranded antiparallel β-sheet incorporating a second loop, and finally a disulphide-bonded flexible third loop.  The C-domain binds IGFs through a largely hydrophobic surface involving the α-helix, the first β-strand, and the first and second loops.
Significance in milk The concentration of IGFBPs is high in bovine prepartum milk-like secretions (BPMS), colostrum and late lactation milk, and low in milk of mid lactation.
PTMs Disulfide bond formation,Glycosylation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions 5 predicted disordered proteins; (12-15),(27-173),(178-182), (188-205), (212-237)
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments Serum levels of IGFBP-6 increase gradually with age and are higher in men than in women
Bibliography 1.Meyer Z, Höflich C, Wirthgen E, Olm S, Hammon HM, Hoeflich A. Analysis of the IGF-system in milk from farm animals - Occurrence, regulation, and biomarker potential. Growth Horm IGF Res. 2017 Aug;35:1-7. doi: 10.1016/j.ghir.2017.05.004. Epub 2017 May 12. PMID: 28544872. 2.Bach LA, Fu P, Yang Z. Insulin-like growth factor-binding protein-6 and cancer. Clin Sci (Lond). 2013 Feb;124(4):215-29. doi: 10.1042/CS20120343. PMID: 23126425.