Search by BoMiProt ID - Bomi6389


Primary Information

BoMiProt ID Bomi6389
Protein Name Inactive serine/threonine-protein kinase VRK3/Serine/threonine-protein pseudokinase VRK3/Vaccinia-related kinase 3
Organism Bos taurus
Uniprot IdQ2YDN8
Milk FractionWhey
Ref Sequence Id NP_001039716.1
Aminoacid Length 451
Molecular Weight 50549
Fasta Sequence https://www.uniprot.org/uniprot/Q2YDN8.fasta
Gene Name VRK3
Gene Id 520302
Protein Existence Status reviewed

Secondary Information

Protein Function It is a direct activator of vaccinia H1-related (VHR) phosphatase, inactivates extracellular signal-regulated kinase (ERK) in the nucleus of neuronal cells.The kinase activity of VRK3 is involved in the regulation of the cell cycle. VRK3 expression levels increase during interphase, whereas VRK1 is enriched in late G2 and early M phase.
Biochemical Properties is catalytically inactive due to changes in motifs that are essential for kinase activity.Has serine/threonine kinase activity,binds to DNA.VRK3 kinase activity is dependent upon its N-terminal regulatory region.VRK3 phosphorylates barrier-to-autointegration factor (BAF) on Ser4.VRK3 contains five potential cyclin-dependent kinase 5 (CDK5) phosphorylation sites, including the repeated motif (K/R)XSPQXT(K/R).
PTMs Phosphorylation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|Q2YDN8|VRK3_BOVIN Inactive serine/threonine-protein kinase VRK3 OS=Bos taurus OX=9913 GN=VRK3 PE=2 SV=1 MICPDCGKGIEATFKFCPYCGKPLPAEKHEGSQSFVKPFTSSSQGSRRKTNTS*53SETS*57SKK VKWCSYAASPSLPLPSEGKS*80S*81GSEDTLS*88TSGKPKGHLSRSPTPRSSPQTTRQSPQTLKRS RMTASLEALPVGTVLTDKSGQHWKLRCLQTRDDQGILYEAESDSTTGSESSPQKQRFSLK LDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSIPQLAIPTCIGFGVHQDKYRFLVFPTLG RSLQSILDDFPKHVMSVRSVFQMACRLLDALEFLHENEYVHGNVTAENIFVNPENLCQVT LAGYGFTFRYSPGGRHVAYTEGSRSPHEGHLEFISMDLHKGCGPSRRSDLQTLGYCLLKW LYGTLPWTNCLPNTEEIVKLKQKFLDNPEGLVGQCSRWITPSETLQEYLKVVMALQYEEK PPYSTLRNELEALLQDLRASAYDPLDLQVVP
Predicted Disorder Regions (20-130), (166-171)
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs oxidative stress-induced cyclin-dependent kinase 5 (CDK5) activation stimulates neuroprotective signaling via phosphorylation of vaccinia-related kinase 3 (VRK3) at Ser 108.The binding of vaccinia H1-related (VHR) phosphatase to phosphorylated VRK3 increased its affinity for phospho-ERK and subsequently downregulated ERK activation.
Bibliography 1.Park CH, Ryu HG, Kim SH, Lee D, Song H, Kim KT. Presumed pseudokinase VRK3 functions as a BAF kinase. Biochim Biophys Acta. 2015 Jul;1853(7):1738-48. doi: 10.1016/j.bbamcr.2015.04.007. Epub 2015 Apr 18. PMID: 25899223. 2.Kang TH, Kim KT. VRK3-mediated inactivation of ERK signaling in adult and embryonic rodent tissues. Biochim Biophys Acta. 2008 Jan;1783(1):49-58. doi: 10.1016/j.bbamcr.2007.10.011. Epub 2007 Nov 4. PMID: 18035061. 3.Song H, Kim W, Choi JH, Kim SH, Lee D, Park CH, Kim S, Kim DY, Kim KT. Stress-induced nuclear translocation of CDK5 suppresses neuronal death by downregulating ERK activation via VRK3 phosphorylation. Sci Rep. 2016 Jun 27;6:28634. doi: 10.1038/srep28634. PMID: 27346674; PMCID: PMC4922050.