Primary Information
BoMiProt ID	Bomi6190
Protein Name	HBS1-like protein
Organism	Bos taurus
Uniprot ID	Q2KHZ2
Milk Fraction	Whey
Ref Sequence ID	NP_001039963.1
Aminoacid Length	686
Molecular Weight	75850
FASTA Sequence	Download
Gene Name	HBS1L
Gene ID	541083
Protein Existence Status	reviewed
Secondary Information
Protein Function	Hbs1 has been established as a central component of the cell's translational quality control pathways in both yeast and prokaryotic models
Biochemical Properties	Hbs1L belongs to a specialized family of translational GTPases (trGTPases), members of which are structurally homologous but functionally distinct [1]. Each trGTPase binds to a specific “decoding” protein and transports it to the ribosomal A site, where it recognizes a unique mRNA code.
PTMs	N6-Acetylation at Lys and Phosphorylation at Ser/Thr
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q2KHZ2|HBS1L_BOVIN HBS1-like protein OS=Bos taurus OX=9913 GN=HBS1L PE=2 SV=1 MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPS*49AVEPVEEYDYE DLKEFSSS*68FVNHQLSGIDQARLYSCLDHMREVLGDAVPDDILIEAVLKNKFDVQKALS*118VV LEQDKVQNLKVRSEGAISTGKIAKGKSIDSQSS*153QS*155ESEIVPKVTKMTVSGKKQTMGFEVP RVTAEENGHSFHTPQKGHSSEDTSLVSSDALESASKSALPSHTIQASEEQSST*233PTPVKKS GKLRQQIDIKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHLLYLLGDVNKRTMHKYEQE SKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETKTKVITLMDAPGHKDFIPNMITG AAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERF QEITGKLGHFLKQAGFKESDVAFIPTSGLSGENLITRSQSSELTKWYKGLCLLEQIDSFK PPQRSIDKPFRLCVSDVFKDQGSGFCVTGKIEAGYIQTGDRLLAMPPNETCTAKGITLHD EPVDWAAAGDHVSLTLVGMDIIKINVGCIFCVPKEPIKVCTRFRARILIFNIEIPITKGF PVLLHYQTVSEPAVIKRLISVLNK*624STGEVTKKKPKLLTKGQNALVELQTQRPVALELYKD FKELGRFMLRYSGSTIAAGVVTEIKD
Predicted Disorder Regions	134-246
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	lack of Hbs1L caused depletion of Pelota protein in both patient cells and mouse tissues, while PELO mRNA levels were unaffected.Hbs1L-deficient human shows facial dysmorphism, growth restriction and retinal deposits.
Bibliography	1.O'Connell AE, Gerashchenko MV, O'Donohue MF, Rosen SM, Huntzinger E, Gleeson D, Galli A, Ryder E, Cao S, Murphy Q, Kazerounian S, Morton SU, Schmitz-Abe K, Gladyshev VN, Gleizes PE, Séraphin B, Agrawal PB. Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation. PLoS Genet. 2019 Feb 1;15(2):e1007917. doi: 10.1371/journal.pgen.1007917. PMID: 30707697; PMCID: PMC6373978. 2.The elongation, termination, and recycling phases of translation in eukaryotes. Dever TE, Green R Cold Spring Harb Perspect Biol. 2012 Jul 1; 4(7):a013706.