Search by BoMiProt ID - Bomi6177


Primary Information

BoMiProt ID Bomi6177
Protein Name Guanylate cyclase soluble subunit alpha-1(GCS-alpha-1)/Soluble guanylate cyclase large subunit
Organism Bos taurus
Uniprot IDP19687
Milk FractionWhey
Ref Sequence ID NP_786972.1
Aminoacid Length 691
Molecular Weight 77533
FASTA Sequence Download
Gene Name GUCY1A1/GUC1A1
Gene ID 281216
Protein Existence Status reviewed

Secondary Information

Protein Function soluble guanylyl cyclase (sGC) is a heterodimeric enzyme with an α and β subunit.role in suppression of VSMC(vascular smooth muscle cells) proliferation and vasorelaxation.
Biochemical Properties alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme.catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate.Responds to Nitric oxide which is a relaxant neurotransmitter.Soluble GC contains a C-terminal catalytic domain, a central dimerization domain, and an N-terminal regulatory domain. existence of an amphipathic alpha-helix in residues 431-464 in the alpha subunit. amphipathicity of the alpha-helix is important for the formation of the heterodimer, and Leu(463) in the alpha-helix region plays a critical role in the formation of a properly arranged active center in the dimer.
PTMs phosphorylation on Ser
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
CATH Matched CATH superfamily
n/a
Predicted Disorder Regions 23-65, 686-691
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs phosphorylation of α1-subunit by protein kinase A enhances the NO-dependent sGC activity, most likely by stabilizing the NO/α1β1 complex. Serine-107A and Serine 108A are the predicted site for phosphorylation.
PDB ID 1AWN,
Bibliography 1.Shiga T, Suzuki N. Amphipathic alpha-helix mediates the heterodimerization of soluble guanylyl cyclase. Zoolog Sci. 2005 Jul;22(7):735-42. doi: 10.2108/zsj.22.735. PMID: 16082162.2.Korkmaz Y, Roggendorf HC, Siefer OG, Seehawer J, Imhof T, Plomann M, Bloch W, Friebe A, Huebbers CU. Downregulation of the α1- and β1-subunit of sGC in Arterial Smooth Muscle Cells of OPSCC Is HPV-Independent. J Dent Res. 2018 Oct;97(11):1214-1221. doi: 10.1177/0022034518774531. Epub 2018 May 18. PMID: 29775416. 3.Kostic TS, Andric SA, Stojilkovic SS. Receptor-controlled phosphorylation of alpha 1 soluble guanylyl cyclase enhances nitric oxide-dependent cyclic guanosine 5'-monophosphate production in pituitary cells. Mol Endocrinol. 2004 Feb;18(2):458-70. doi: 10.1210/me.2003-0015. Epub 2003 Nov 20. PMID: 14630997.