Primary Information |
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BoMiProt ID | Bomi6059 |
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Protein Name | Glycerol-3-phosphate dehydrogenase, mitochondrial/GPD-M/GPDH-M |
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Organism | Bos taurus |
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Uniprot ID | A6QLU1 |
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Milk Fraction | Whey,MFGM |
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Ref Sequence ID | NP_001093766.1 |
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Aminoacid Length | 727 |
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Molecular Weight | 80763 |
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FASTA Sequence |
Download |
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Gene Name | GPD2 |
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Gene ID | 504948 |
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Protein Existence Status | reviewed |
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Secondary Information |
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Protein Function | a component of the glycerol phosphate shuttle, boosted glucose oxidation to fuel the production of acetyl-coA, acetylation of histones and induction of genes encoding inflammatory mediators.The main metabolic role of this shuttle is reoxidation of cytosolic NADH produced by glycolysis.This enables sustained rate of cytosolic ATP production without accumulation of an intermediate by-product such as lactic acid. |
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Biochemical Properties | Calcium-binding enhance the activity of the enzyme.binds to FAD as cofactor.that oxidizes glycerol-3-phosphate (G3P) to dihdroxy acetone phosphate(DHAP).Kinetic properties of isolated mGPDH revealed 2.9 mM Km for G3P and ~ 7 μM Km for FAD in rat liver enzyme |
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PTMs | Proteolytic cleavage,Phosphorylation on Tyr |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A6QLU1|GPDM_BOVIN Glycerol-3-phosphate dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=GPD2 PE=2 SV=1
MAFQKAVKGTILVGGGALATVLGLSHFAHYKRKQVNLAFVEAADCISEPVNREPPSREAQ
ILTLKNTSEFDVLVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVR
YLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPIYKWWQLPYYWVGIKLY
DLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYG
AATANYMEVMSLLKKTDPQTGKERVSGARCKDVLTGEEFDVRAKCVINATGPFTDTVRKM
DDKDTTAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGTTDTPTD
VTHHPIPSEEDINFILNEVRNYLSCDVEVRRGDVLAAWSGIRPLVTDPKSADTKSISRNH
VVDISESGLITIAGGKWTTYRSMAEDTINAAVKAHNLKAGPSRTVGLFLQGGKDWSPTLY
IRLVQDYGLESEVAQHLAATYGDKAFEVAKMASVTGKRWPIVGVRLVSEFPYIEAEVKYG
IKEYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNWDDSKKEEELETARKFLY
Y*601EMGYKSRSEQLTDRSEISLLPSDIDRYKKRFHKFDADQKGFITIVDVQRVLESIGVQMD
ENTLHEILNEVDLNKNGQVELNEFLQLMSAIQKGRVSGSRLAILMKTAEENLDRRVPIPV
DRSCGGL |
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Predicted Disorder Regions | NA |
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DisProt Annotation | |
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TM Helix Prediction | 1TMH; (11-29) |
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Significance of PTMs | Proteolytic digestion-Rat mGPDH is synthesized as 79 kDa precursor that is processed to ~ 74 kDa mature protein upon removal of the N terminal(5kDa) presequence,after import into the mitochondria. |
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Bibliography | Langston PK, Nambu A, Jung J, Shibata M, Aksoylar HI, Lei J, Xu P, Doan MT, Jiang H, MacArthur MR, Gao X, Kong Y, Chouchani ET, Locasale JW, Snyder NW, Horng T. Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses. Nat Immunol. 2019 Sep;20(9):1186-1195. doi: 10.1038/s41590-019-0453-7. Epub 2019 Aug 5. Erratum in: Nat Immunol. 2019 Sep 24;: PMID: 31384058; PMCID: PMC6707851. |