Search by BoMiProt ID - Bomi6037


Primary Information

BoMiProt ID Bomi6037
Protein Name Glutamine-dependent NAD(+) synthetase/NAD(+) synthetase
Organism Bos taurus
Uniprot IdQ3ZBF0
Milk FractionExosomes
Ref Sequence Id NP_001029615.1
Aminoacid Length 706
Molecular Weight 79400
Fasta Sequence https://www.uniprot.org/uniprot/Q3ZBF0.fasta
Gene Name NADSYN1
Gene Id 513400
Protein Existence Status Reviewed

Secondary Information

Presence in other biological fluids/tissue/cells pituitary gland
Protein Function NAD(+) synthetase catalyzes the formation of NAD(+) from ATP, nicotinic acid adenine dinucleotide and ammonia. Glutamine-dependent NAD(+) synthetase obtains ammonia through the hydrolysis of glutamine to glutamate, which takes place in the glutaminase domain. 
Biochemical Properties Glutamine-dependent NAD(+) synthetase, Qns1, utilizes a glutamine aminotransferase domain to supply ammonia for amidation of nicotinic acid adenine dinucleotide (NaAD(+)) to NAD(+). 
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions 700-706
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments  Six distinct classes of Qns1 mutants that fall within the glutaminase domain and the synthetase domain selectively inhibit components of the coordinated reaction.
Bibliography 1.Resto M, Yaffe J, Gerratana B. An ancestral glutamine-dependent NAD(+) synthetase revealed by poor kinetic synergism. Biochim Biophys Acta. 2009 Nov;1794(11):1648-53. doi: 10.1016/j.bbapap.2009.07.014. Epub 2009 Aug 6. PMID: 19647806. 2.Wojcik M, Seidle HF, Bieganowski P, Brenner C. Glutamine-dependent NAD+ synthetase. How a two-domain, three-substrate enzyme avoids waste. J Biol Chem. 2006 Nov 3;281(44):33395-402. doi: 10.1074/jbc.M607111200. Epub 2006 Sep 5. PMID: 16954203.