Primary Information
BoMiProt ID	Bomi5383
Protein Name	E3 ubiquitin-protein ligase SH3RF1/Protein POSH/RING-type E3 ubiquitin transferase SH3RF1/SH3 domain-containing RING finger protein 1
Organism	Bos taurus
Uniprot ID	A5D7F8
Milk Fraction	Whey
Ref Sequence ID	NP_001091534.1
Aminoacid Length	840
Molecular Weight	87592
FASTA Sequence	Download
Gene Name	SH3RF1/POSH/ POSH1
Gene ID	528315
Protein Existence Status	Reviewed
Secondary Information
Protein Function	Has E3 ubiquitin-protein ligase activity. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis.
PTMs	Phosphorylation
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|A5D7F8|SH3R1_BOVIN E3 ubiquitin-protein ligase SH3RF1 OS=Bos taurus OX=9913 GN=SH3RF1 PE=2 SV=1 MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGV EQLPSNILLVRLLDGIKQRPWKPGPVGGSGTNGTSALRAQSSAVVTCSPKDGPSSQGGPQ PRAQAWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIVLRRQVDENWYHGEVGGVH GFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEG MLADKIGIFPISYVEFNSAAKQLIEWDQPPGPGVAAGEGALATTPSSTTTKQPDGKKNTK KRHS*304FTSLSMASKASQAAQQRHSMEISPPVLISSSNPAAAARIGELAGLSCSAPSQVHIS TTGLIVTPPPSSPVTTGPSFTFPAEAPYPAALATLNPPLPPPPLQAATPTGTAVAAAAGM GPRPTAGPTDQTTHPRPQPRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWFKGT SMHTSKIGVFPGNYVAPVTRAVTSASQGKVPMLTTGPASRGGVLANPPSTGGPAQKPPGN GVAGGPGVPTAVVSAAHVQTSPQAKVLLHASGQMTVNQARSAARTVSAHSQERPTAAVTP IQVQSTPGQSHHPLVSPQPPAPLGPPAHAAASGLGRVGGPLACATAPASIPAASLEPEPS SRPATLLPGTPTSPDSGSAARPDKDGKKEKKGLLKLLSGASTKRKPRGS*709PPASPTLDAEL GAELSCGPPGPPCACPGPCDGDTMAPGPQRRASSLDSAPVAPPPRQPCSSLGPAASEVRP AVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Significance of PTMs	Phosphorylated at Ser-304 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac
Bibliography	Tuvia, S., Taglicht, D., Erez, O., Alroy, I., Alchanati, I., Bicoviski, V., Dori-Bachash, M., Ben-Avraham, D., & Reiss, Y. (2007). The ubiquitin E3 ligase POSH regulates calcium homeostasis through spatial control of Herp. The Journal of cell biology, 177(1), 51–61. https://doi.org/10.1083/jcb.200611036