Search by BoMiProt ID - Bomi5378


Primary Information

BoMiProt ID Bomi5378
Protein Name E3 ubiquitin-protein ligase RNF8/RING finger protein 8/RING-type E3 ubiquitin transferase RNF8
Organism Bos taurus
Uniprot IdQ2HJ46
Milk FractionWhey
Ref Sequence Id NP_001039681.1
Aminoacid Length 487
Molecular Weight 55729
Fasta Sequence https://www.uniprot.org/uniprot/Q2HJ46.fasta
Gene Name RNF8
Gene Id 515933
Protein Existence Status reviewed

Secondary Information

Protein Function The ubiquitin ligase RNF8 is known to induce epithelial-to-mesenchymal (EMT) transition and metastasis in triple-negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis.
Biochemical Properties The E3 ligase RNF8 ubiquitylates histone H1 at DSB flanking sites, triggering an orchestrated recruitment of signaling and repair proteins (e.g., the breast cancer susceptibility protein BRCA1) to facilitate the repair of the break sites
PTMs Phosphorylation at Serine
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|Q2HJ46|RNF8_BOVIN E3 ubiquitin-protein ligase RNF8 OS=Bos taurus OX=9913 GN=RNF8 PE=2 SV=1 MGDPGSLVTEGRAGERSWCLRRVGMNTEWLLLEDGNEVTVGRGFGVTYQLVSKICPLMIS RNHCILKQNAEGQWTIKDNKSLNGVWLNRERLEPLKVYSIHKGDHIQLGVPLENKENAEY EYEVTEEDWERIYPCLSPKSDQMMEKNKGLRTKRKFS*157LDELEGSGAEGPSNLKSKISKLS CEPGQQVKSHGKGKVASQPSEYLDPKLTSFEPSVKTTGAHVNPGPAKVIELLRKKKKASN PSASQSSLELFKVTMSRILMLKTQMQEKQVAVLNVKKQTKKGSSKKIVKMEQELQDLQSQ LCAEQAQQQARVEQLEKTIQEEQQHLEGLEKEEGEEDLKQQLAQALQEYRSLVEELNRSK KNFEAIIQAKDKELEQTKEEKEKVQAQKEEVLSHMNDVLENELQCIICSEYFVEAVTLNC AHSFCSYCINEWMKRKVECPICRKDIKSKTRSLVLDNCISKMVDNLNSEVKERRIVLIRE RKGKRLF
Predicted Disorder Regions 4-11, 137-246, 276-338
DisProt Annotation
TM Helix Prediction no TM helices
Significance of PTMs Autoubiquitination on Lys-48 and 63 of ubiquitin.Polyubiquitination at Lys-29 is also observed, but it doesn't require its own functional RING-type zinc finger.
Additional Comments The E3 ubiquitin ligase RNF8 plays critical roles in maintaining genomic stability by promoting the repair of DNA double-strand breaks (DSBs) through ubiquitin signaling. Abnormal activation of Notch signaling and defective repair of DSBs promote breast cancer risk.
Bibliography 1.Pereira De Carvalho B, Chern YJ, He J, Chan CH. The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple-negative breast cancer cells. FEBS Lett. 2021 Jan;595(2):241-252. doi: 10.1002/1873-3468.13999. Epub 2020 Dec 5. PMID: 33205415; PMCID: PMC7898409. 2.RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly. Huen MS, Grant R, Manke I, Minn K, Yu X, Yaffe MB, Chen J Cell. 2007 Nov 30; 131(5):901-14. 3.Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, Narita T, Sixma TK, Choudhary C, Bekker-Jensen S, Mailand N Nature. 2015 Nov 19; 527(7578):389-93.