Search by BoMiProt ID - Bomi5374


Primary Information

BoMiProt ID Bomi5374
Protein Name E3 ubiquitin-protein ligase RNF25
Organism Bos taurus
Uniprot IdQ5E9N3
Milk FractionWhey
Ref Sequence Id NP_001015575.1
Aminoacid Length 458
Molecular Weight 51236
Fasta Sequence https://www.uniprot.org/uniprot/Q5E9N3.fasta
Gene Name RNF25
Gene Id 511571
Protein Existence Status reviewed

Secondary Information

Protein Function is an E3 ubiquitin ligase targets Nkd2 for degradation,physically interacts with Nkd1 and Axin in an E3 ligase-independent manner to strengthen Wnt signalling.Stimulates transcription mediated by NF-kappa-B and EGF Receptor (EGFR) signaling.
Biochemical Properties The interaction with Nkd2 is mediated by the RING domain (aa 113–291), a domain that is essential for its E3 ubiquitin ligase activity, leading to Nkd2 polyubiquitination and degradation.Also C-terminal Proline rich region (aa 292–513) is imp for interaction.
PTMs Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions 1-7, 174-189, 218-332, 351-375, 397-406, 452-458
DisProt Annotation
TM Helix Prediction no TM helices
Bibliography 1.Gao R, Ma LQ, Du X, Zhang TT, Zhao L, Liu L, Liu JC, Guo F, Cheng Z, Huang H. Rnf25/AO7 positively regulates wnt signaling via disrupting Nkd1-Axin inhibitory complex independent of its ubiquitin ligase activity. Oncotarget. 2016 Apr 26;7(17):23850-9. doi: 10.18632/oncotarget.8126. PMID: 27007149; PMCID: PMC5029668.