Search by BoMiProt ID - Bomi5242


Primary Information

BoMiProt ID Bomi5242
Protein Name Dolichol phosphate-mannose biosynthesis regulatory protein/Dolichol-phosphate mannose synthase subunit 2/DPM synthase subunit 2
Organism Bos taurus
Uniprot IdQ2KIN1
Milk FractionWhey,MFGM
Ref Sequence Id NP_001039733.1
Aminoacid Length 84
Molecular Weight 9395
Fasta Sequence https://www.uniprot.org/uniprot/Q2KIN1.fasta
Gene Name DPM2
Gene Id 523737
Protein Existence Status reviewed

Secondary Information

Protein Function Dolichol-phosphate-mannose (DPM) synthase generates mannosyl donors for glycosylphosphatidylinositols, N-glycan and protein O- and C-mannosylation.DPM2 stabilizes DPM3 and DPM3 stabilizes DPM1. DPM synthase activity was 10 times higher in the presence of DPM2, indicating that DPM2 also plays a role in the enzymatic reaction.
Biochemical Properties Mammalian DPM synthase contains catalytic DPM1 and regulatory DPM2 subunits, and that DPM1 requires DPM2 for its stable expression in the endoplasmic reticulum. Human DPM synthase consists of three subunits. The third subunit, DPM3, comprises 92 amino acids associated with DPM1 via its C-terminal domain and with DPM2 via its N-terminal portion. The stability of DPM3 was dependent upon DPM2.
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
na
Predicted Disorder Regions 1-4, 78-84
DisProt Annotation
TM Helix Prediction 2TMHs; (13-31), (50-72)
Additional Comments Component of the dolichol-phosphate mannose (DPM) synthase complex composed of DPM1, DPM2 and DPM3; in the complex interacts directly with DPM3.Component of the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex composed at least by PIGA, PIGC, PIGH, PIGP, PIGQ, PIGY and DPM2.
Bibliography 1.Maeda Y, Tanaka S, Hino J, Kangawa K, Kinoshita T. Human dolichol-phosphate-mannose synthase consists of three subunits, DPM1, DPM2 and DPM3. EMBO J. 2000 Jun 1;19(11):2475-82. doi: 10.1093/emboj/19.11.2475. PMID: 10835346; PMCID: PMC212771.