Search by BoMiProt ID - Bomi5217


Primary Information

BoMiProt ID Bomi5217
Protein Name DNA-directed RNA polymerase III subunit RPC1/RNA polymerase III subunit C1/DNA-directed RNA polymerase III subunit A
Organism Bos taurus
Uniprot IdA4IF62
Milk FractionWhey
Ref Sequence Id NP_001077219.1
Aminoacid Length 1390
Molecular Weight 155791
Fasta Sequence https://www.uniprot.org/uniprot/A4IF62.fasta
Gene Name POLR3A
Gene Id 540308
Protein Existence Status reviewed

Secondary Information

Protein Function POLR3A and POLR3B encode the largest and second largest subunits of RNA Polymerase III (Pol III), RPC1 and RPC2, respectively. RPC1 and RPC2 together form the active center of the polymerase and contribute to the catalytic activity of the polymerase. Pol III is involved in the transcription of small noncoding RNAs, such as 5S ribosomal RNA and all transfer RNAs (tRNA).
PTMs N6-Acetylation at Lys
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
na
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments Overexpression of the S.cerevisiae largest subunit of RNA polymerase III C160 (Rpc1) allows S.pombe rpb8 to functionally replace ABC14.5 in S.cerevisiae, suggesting a specific genetic interaction between the S.cerevisiae ABC14.5 (Rpb8) and C160 subunits. 
Bibliography 1.Saitsu H, Osaka H, Sasaki M, Takanashi J, Hamada K, Yamashita A, Shibayama H, Shiina M, Kondo Y, Nishiyama K, Tsurusaki Y, Miyake N, Doi H, Ogata K, Inoue K, Matsumoto N. Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy. Am J Hum Genet. 2011 Nov 11;89(5):644-51. doi: 10.1016/j.ajhg.2011.10.003. Epub 2011 Oct 27. PMID: 22036171; PMCID: PMC3213392. 2.Voutsina A, Riva M, Carles C, Alexandraki D. Sequence divergence of the RNA polymerase shared subunit ABC14.5 (Rpb8) selectively affects RNA polymerase III assembly in Saccharomyces cerevisiae. Nucleic Acids Res. 1999 Feb 15;27(4):1047-55. doi: 10.1093/nar/27.4.1047. PMID: 9927738; PMCID: PMC148285.