Search by BoMiProt ID - Bomi5193


Primary Information

BoMiProt ID Bomi5193
Protein Name DNA polymerase beta
Organism Bos taurus
Uniprot IdQ27958
Milk FractionWhey
Ref Sequence Id NP_001029936.1
Aminoacid Length 335
Molecular Weight 38267
Fasta Sequence https://www.uniprot.org/uniprot/Q27958.fasta
Gene Name POLB
Gene Id 614688
Protein Existence Status reviewed

Secondary Information

Protein Function key role in base-excision repair. 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap.
Biochemical Properties contains lyase and polymerase domains.The polymerase domain is composed of the C- (Catalytic), D- (Duplex DNA binding), and N-subdomains (Nascent base pair binding).
PTMs Acetylation, Isopeptide bond formation, Methylation, Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
na
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.Ubiquitinated at Lys-41, Lys-61 and Lys-81,leading to degradation by the proteasome.
Bibliography 1.Beard WA. DNA polymerase β: Closing the gap between structure and function. DNA Repair (Amst). 2020 Sep;93:102910. doi: 10.1016/j.dnarep.2020.102910. PMID: 33087276; PMCID: PMC7643811.