Search by BoMiProt ID - Bomi5191


Primary Information

BoMiProt ID Bomi5191
Protein Name DNA nucleotidylexotransferase/Terminal addition enzyme/Terminal deoxynucleotidyltransferase/TDT/Terminal transferase
Organism Bos taurus
Uniprot IdP06526
Milk FractionWhey
Ref Sequence Id NP_803461.1
Aminoacid Length 509
Molecular Weight 58296
Fasta Sequence https://www.uniprot.org/uniprot/P06526.fasta
Gene Name DNTT/TDT
Gene Id 281120
Protein Existence Status reviewed

Secondary Information

Protein Function role in the initiation step of DNA replication.Template-independent DNA polymerase which catalyzes the addition of deoxynucleoside 5'-triphosphate to the 3'-end of oligonucleotide primers.
Biochemical Properties Cofactor is Mg2+.The bovine TdT cDNA clone contains an open reading frame coding for 520 amino acids, Mr 59,678.nuclear-localization-sequence (Pro-Arg-Lys-Lys-Arg-Pro-Arg) was conserved in the N-terminal region in the mouse and bovine cDNA clones.
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
na
Predicted Disorder Regions 1-27, 135-144, 413-419
DisProt Annotation
TM Helix Prediction No TM helices
Bibliography Koiwai O, Yokota T, Kageyama T, Hirose T, Yoshida S, Arai K. Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells. Nucleic Acids Res. 1986 Jul 25;14(14):5777-92. doi: 10.1093/nar/14.14.5777. PMID: 3755527; PMCID: PMC311591.