Search by BoMiProt ID - Bomi4731


Primary Information

BoMiProt ID Bomi4731
Protein Name Clathrin heavy chain 1
Organism Bos taurus
Uniprot IDP49951
Milk FractionWhey
Ref Sequence ID NP_776448.1
Aminoacid Length 1675
Molecular Weight 191589
FASTA Sequence Download
Gene Name CLTC
Gene ID 281080
Protein Existence Status reviewed

Secondary Information

Protein Function Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep
Biochemical Properties The N-terminal domain ~NTD! of clathrin heavy chain 1 ~CLTC! is important for clathrin to bind to the spindle, and studies indicate that NTD and the trimerization domain of CTLC are essential for its mitotic function
PTMs Phosphorylation at Ser/Thr
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|P49951|CLH1_BOVIN Clathrin heavy chain 1 OS=Bos taurus OX=9913 GN=CLTC PE=1 SV=1 MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSN PIRRPIS*67ADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMT*105DDVTFWKWISLNTVA LVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGA MQLY*184SVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGN QPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISG ETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGA EELFARKFNALFAQGNYSEAAKVAANAPKGILRT*394PDTIRRFQSVPAQPGQTSPLLQYFGI LLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYL RANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDE EPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAIL GNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLY*634DIKRAVVHTHLLNPEWLVNYFGSLSV EDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVN FSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDR FDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQF STDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPY*899Y DSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVL LESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSE HRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTS AVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEV VQAANTSGNWEELVKYLQMARKKARES*1167YVETELIFALAKTNRLAELEEFINGPNNAHIQQ VGDRCY*1206DEKMYDAAKLLYNNVSNFGRLAS*1229TLVHLGEYQAAVDGARKANSTRTWKEVCFAC VDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTEL AILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMN HPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFS KVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDY*1477QALRTSIDAY*1487DNFDNIS*1494LAQRLE KHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQE EKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLR KEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPAYGQPQPGFGYSM
SCOP Class : All alpha proteins
Fold : alpha-alpha superhelix
Superfamily : ARM repeat-like
Family : Clathrin heavy chain proximal leg segment
Domain Name : 1B89 A:1182-1517

Class : All alpha proteins
Fold : alpha-alpha superhelix
Superfamily : ARM repeat-like
Family : Clathrin heavy-chain linker domain
Domain Name : 1UTC A:331-355

Class : All beta proteins
Fold : 7-bladed beta-propeller
Superfamily : Clathrin heavy-chain terminal domain-like
Family : Clathrin heavy-chain terminal domain
Domain Name : 1UTC A:4-330

CATH Matched CATH superfamily
1.25.40.10
1.25.40.730
2.130.10.110
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
PDB ID 1B89,1UTC,1XI4,1XI5,3GC3,3GD1,3IYV,3LVG,3LVH,3QIL,5M5R,5M5S,5M5T,5M5U,5M5V,5M61,6WCJ,
Additional Comments CLTC plays important roles in both spindle assembly and chromosome congression by interacting with cytoskeleton-associated protein 5 (CKAP5)
Bibliography 1.Han Z, Hao X, Zhou CJ, Wang J, Wen X, Wang XY, Zhang DJ, Liang CG. Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep. Front Cell Dev Biol. 2021 Feb 25;9:609311. doi: 10.3389/fcell.2021.609311. PMID: 33718352; PMCID: PMC7946971. 2.Han, Z., Liang, C. G., Cheng, Y., Duan, X., Zhong, Z., Potireddy, S., et al. (2010). Oocyte spindle proteomics analysis leading to rescue of chromosome congression defects in cloned embryos. J. Proteome Res. 9, 6025–6032. doi: 10.1021/pr100827j. 3.Royle, S.J. & Lagnado, L. ~2006!. Trimerisation is important for the function of clathrin at the mitotic spindle. J Cell Sci 119~Pt 19!, 4071–4078.