Search by BoMiProt ID - Bomi41

Primary Information

BoMiProt ID Bomi41
Protein Name Annexin A1
Organism Bos taurus
Uniprot IdP46193
Milk FractionMFGM, Exosome
Ref Sequence Id NP_786978.2
Amino Acid Lenth 346
Molecular Weight 38952
Fasta Sequence
Gene Name ANXA1
Gene Id 327662
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondry Information

Protein Function annexin I aggregates membrane phospholipids; suppress phospholipase A2 activity; inhibits gland differentiation
Biochemical Properties Cytosolic annexin I had a molecular mass of 36 kDa which was also found in the nuclear fraction; A 38-kDa annexin I was detected in nuclei; The cytosolic and nuclear 36-kDa annexin I and the nuclear 38-kDa annexin I showed different isoelectric points; induced by glucocorticoids
Significance in milk increased glucocorticoid concentration induces annexin A1, which is required for the initiation of lactation
PTMs phosphorylated by pkc as found in bocine mammary gland; glycosylated as found in human placenta - presence of N-acetyl-D-galactosamine residues, two cc-linked biantennary mannosyl residues, one site of asparagine-linked glycosylation which may contain two a-linked biantennary mannosyl residues; indicates a potential Nlinked glycosylation site at the 42nd position of its amino terminal region
Significance of PTMs Phosphorylation by protein kinase C changes the pI of annexin I; Phosphorylation by protein kinase C inhibits the abilities of unphosphorylated annexin I to aggregate membrane phospholipids and to suppress phospholipase A2 activity
Additional Comments Annexin I and annexin II can then be easily separated from each other by Mono S fast protein liquid chromatography
Bibliography 1. Katoh, N., Suzuki, T., Yuasa, A., & Miyamoto, T. (1995). Distribution of annexins I, II, and IV in bovine mammary gland. Journal of Dairy Science, 78(11), 2382–2387.
2. Goulet, F., Moore, K. G., & Sartorelli, A. C. (1992). Glycosylation of annexin I and annexin II. Biochemical and Biophysical Research Communications, 188(2), 554–558.