Primary Information |
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| BoMiProt ID | Bomi3928 |
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| Protein Name | Ankyrin repeat domain-containing protein 54 |
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| Organism | Bos taurus |
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| Uniprot ID | Q1LZC5 |
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| Milk Fraction | whey |
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| Ref Sequence ID | NP_001069605.1 |
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| Aminoacid Length | 299 |
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| Molecular Weight | 32466 |
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| FASTA Sequence |
Download |
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| Gene Name | ANKRD54 |
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| Gene ID | 538961 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Protein Function | Ankyrin repeat domain-containing protein 54 plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. |
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| Biochemical Properties | a novel nuclear-cytoplasmic (containing functional NLS and NES motifs) shuttling adaptor of the Src family tyrosine kinase Lyn and other signalling molecules (e.g., HCLS1), that regulates down-stream signalling in erythropoietin-responsive erythroid cells[ |
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| PTMs | Acetylation and phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q1LZC5|ANR54_BOVIN Ankyrin repeat domain-containing protein 54 OS=Bos taurus OX=9913 GN=ANKRD54 PE=2 SV=1
MAAAAGGADDESRSGRSSSDGECAVAPEPLTGPEGLFSFADFGSALGGGAGLPGRAS*57GGA
QS*62PLRYLHVLWQQDAEPRDELRCKIPAGRLRRAARPHRRLGPTGKEVHALKRLRDSANAN
DVETVQQLLEEGTDPCAADDKGRTALHFASCNGNDQIVQLLLDHGADPNQRDGLGNTPLH
LAACTNHAPVITTLLRGGARVDALDRAGRTPLHLAKSKLNILQEGHSQCLEAVRLEVKQI
IQMLREYLERLGRHEQRERLDDLCTRLQKTSTREQVDEVTDLLASFTSLSLQMQNMEKR
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| Predicted Disorder Regions | 1-58, 100-105 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | phosphorylation by PKCδ as a major regulator of nuclear-cytoplasmic shuttling of Ankrd54.Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn. Co-expression of an active form of the PKCδ isoform specifically promoted both phosphorylation and cytoplasmic localization of Ankrd54. Alanine mutation of several serine residues in the amino-terminal region of Ankrd54 reduced both phorbol 12-myristate 13-acetate induced cytoplasmic localization and phosphorylation of Ankrd54.PKCδ as a major regulator of nuclear-cytoplasmic shuttling and interaction of Ankrd54 with Lyn, through its phosphorylation of at least the Ser18 residue. |
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| Bibliography | 1.Samuels AL, Louw A, Zareie R, Ingley E. Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ. World J Biol Chem. 2017 Aug 26;8(3):163-174. doi: 10.4331/wjbc.v8.i3.163. PMID: 28924458; PMCID: PMC5579962. 2.Samuels, A. L., Louw, A., Zareie, R., & Ingley, E. (2017). Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ. World journal of biological chemistry, 8(3), 163–174. https://doi.org/10.4331/wjbc.v8.i3.163 |
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