Primary Information
BoMiProt ID	Bomi3631
Protein Name	Activated CDC42 kinase 1/ACK-1/Activated CDC42 kinase 2/Tyrosine kinase non-receptor protein 2
Organism	Bos taurus
Uniprot Id	Q17R13
Milk Fraction	whey
Ref Sequence Id	NP_776310.2
Aminoacid Length	1039
Molecular Weight	114868
Fasta Sequence	https://www.uniprot.org/uniprot/Q17R13.fasta
Gene Name	TNK2/ACK1/ACK2
Gene Id	280710
Protein Existence Status	Reviewed
Secondary Information
Protein Function	Involved in ell spreading and migration, cell survival, cell growth and proliferation.It binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs.
Biochemical Properties	contains (from N to C terminus) the kinase catalytic domain, SH3 domain, and Cdc42-binding Cdc42/Rac interactive binding (CRIB) domain followed by a proline rich domain.binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain.Catalyses the following reaction- ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]
PTMs	Phosphorylation,Ubl conjugation
Significance of PTMs	autophosphorylation on Tyr284 enhances kinase activity.ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) via its C-terminal proline-rich domain.
Linking IDs	Bomi3631
Bibliography	Yokoyama N, Miller WT. Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck. J Biol Chem. 2003 Nov 28;278(48):47713-23. doi: 10.1074/jbc.M306716200. Epub 2003 Sep 22. PMID: 14506255.
Protein Function	Involved in ell spreading and migration, cell survival, cell growth and proliferation.It binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs.
Biochemical Properties	contains (from N to C terminus) the kinase catalytic domain, SH3 domain, and Cdc42-binding Cdc42/Rac interactive binding (CRIB) domain followed by a proline rich domain.binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain.Catalyses the following reaction- ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]
PTMs	Phosphorylation,Ubl conjugation
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q17R13|ACK1_BOVIN Activated CDC42 kinase 1 OS=Bos taurus OX=9913 GN=TNK2 PE=1 SV=1 MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLW EAVKRRKAMCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPTPGGSAGEGSLQSLTCL IGEKDLHLFEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNA MHSLDHRNLIRLYGVVLTPPMKTVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGM GYLEAKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHY*284VMQEHRKVPFAWCAPE SLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDI YNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRA ENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPD KIDELYLGNPMDPPDLLSVELSTSRPTQHLGRVKKPTY*518DPVSEDQDPLSSDFKRLGLRKP GLPRGLWLAKPSARVPGTKAGRGGGEVTLIDFGEEPVVPAPRPCAPSLAQLAMDACSLLD KTPPQSPTRALPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDFEVCSINSTLVGAGVSA EPSQGETNYAFVPEPARLLPPLEDNLFLPPQSGGKPPNSAQTAEIFQALQQECMRQLQVP PGS*723LVPSPSPGGDDKPQVPPRVPIPPRPTRSRGELSPVPPGEEEMGRWPGPASPPRVPPR EPLSPQGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQSFASDPKY*826ATPQVIQAPGPRAG PCILPIVRDGKKVSSTHY*858YLLPERPPYLERY*871QRFLHEAQS*880PRGPDPTPIPLLLPPPSTPA PAAPTATVRPMPQAAPDPKANFSSNNSNPGARPSSLRATARLPQRGYPGDGPEAGRPADK IQMLQAMVHGVTTEECQAALQSHSWSVQRAAQYLKVEQLFGLGLRPRGECHNVLEMFDWN LEQAGCHLLGSCGPAHHKR
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Significance of PTMs	autophosphorylation on Tyr284 enhances kinase activity.ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) via its C-terminal proline-rich domain.
Linking IDs
Bibliography	Yokoyama N, Miller WT. Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck. J Biol Chem. 2003 Nov 28;278(48):47713-23. doi: 10.1074/jbc.M306716200. Epub 2003 Sep 22. PMID: 14506255.