Search by BoMiProt ID - Bomi3549


Primary Information

BoMiProt ID Bomi3549
Protein Name 60S ribosomal protein L18
Organism Bos taurus
Uniprot IdQ5E973
Milk FractionWhey,MFGM
Ref Sequence Id NP_001015556.1
Aminoacid Length 188
Molecular Weight 21535
Fasta Sequence https://www.uniprot.org/uniprot/Q5E973.fasta
Gene Name RPL18
Gene Id 509163
Protein Existence Status reviewed

Secondary Information

Protein Function Component of the large ribosomal subunit.L18 inhibited both PKR autophosphorylation and PKR-mediated phosphorylation of eIF-2alpha thus prevents polypeptide chain initiation. In such a manner, activated PKR inhibits cell growth and induces apoptosis.
Biochemical Properties RNA binding activity. L18 is a 22-kDa protein
PTMs Removal of Methionine,phosphorylation on Ser and Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|Q5E973|RL18_BOVIN 60S ribosomal protein L18 OS=Bos taurus OX=9913 GN=RPL18 PE=2 SV=3 MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPP LSLSRMIRKMKLPGREGKTAVVVGTITDDVRVQEVPKLKVCALRVSSRARSRILKAGGKI LTFDQLALDS*130PKGCGTVLLSGPRKGREVYRHFGKAPGT*158PHSHTKPYVRSKGRKFERARGR RASRGYKN
Predicted Disorder Regions 1-28, 63-78, 100-113, 150-188
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs In E coli L18,removal of Methionine from N terminal by methionine aminopeptidase (MAP).The 2nd residue following Met is Asp.
Bibliography 1.Nesterchuk MV, Sergiev PV, Dontsova OA. Posttranslational Modifications of Ribosomal Proteins in Escherichia coli. Acta Naturae. 2011 Apr;3(2):22-33. PMID: 22649682; PMCID: PMC3347575. 2.Kumar KU, Srivastava SP, Kaufman RJ. Double-stranded RNA-activated protein kinase (PKR) is negatively regulated by 60S ribosomal subunit protein L18. Mol Cell Biol. 1999 Feb;19(2):1116-25. doi: 10.1128/MCB.19.2.1116. PMID: 9891046; PMCID: PMC116041.