Search by BoMiProt ID - Bomi3549


Primary Information

BoMiProt ID Bomi3549
Protein Name 60S ribosomal protein L18
Organism Bos taurus
Uniprot IDQ5E973
Milk FractionWhey,MFGM
Ref Sequence ID NP_001015556.1
Aminoacid Length 188
Molecular Weight 21535
FASTA Sequence Download
Gene Name RPL18
Gene ID 509163
Protein Existence Status reviewed

Secondary Information

Protein Function Component of the large ribosomal subunit.L18 inhibited both PKR autophosphorylation and PKR-mediated phosphorylation of eIF-2alpha thus prevents polypeptide chain initiation. In such a manner, activated PKR inhibits cell growth and induces apoptosis.
Biochemical Properties RNA binding activity. L18 is a 22-kDa protein
PTMs Removal of Methionine,phosphorylation on Ser and Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|Q5E973|RL18_BOVIN 60S ribosomal protein L18 OS=Bos taurus OX=9913 GN=RPL18 PE=2 SV=3 MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPP LSLSRMIRKMKLPGREGKTAVVVGTITDDVRVQEVPKLKVCALRVSSRARSRILKAGGKI LTFDQLALDS*130PKGCGTVLLSGPRKGREVYRHFGKAPGT*158PHSHTKPYVRSKGRKFERARGR RASRGYKN
Predicted Disorder Regions 1-28, 63-78, 100-113, 150-188
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs In E coli L18,removal of Methionine from N terminal by methionine aminopeptidase (MAP).The 2nd residue following Met is Asp.
Bibliography 1.Nesterchuk MV, Sergiev PV, Dontsova OA. Posttranslational Modifications of Ribosomal Proteins in Escherichia coli. Acta Naturae. 2011 Apr;3(2):22-33. PMID: 22649682; PMCID: PMC3347575. 2.Kumar KU, Srivastava SP, Kaufman RJ. Double-stranded RNA-activated protein kinase (PKR) is negatively regulated by 60S ribosomal subunit protein L18. Mol Cell Biol. 1999 Feb;19(2):1116-25. doi: 10.1128/MCB.19.2.1116. PMID: 9891046; PMCID: PMC116041.