Search by BoMiProt ID - Bomi352


Primary Information

BoMiProt ID Bomi352
Protein Name D-3-phosphoglycerate dehydrogenase
Organism Bos taurus
Uniprot IdQ5EAD2
Milk FractionExosome
Ref Sequence Id NP_001030189.1
Aminoacid Length 533
Molecular Weight 56452
Fasta Sequence https://www.uniprot.org/uniprot/Q5EAD2.fasta
Gene Name PHGDH
Gene Id 505103
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function catalyzes the first step in the pathway by converting D-3- phosphoglycerate (PGA), an intermediate in glycolysis, to phosphohydroxypyruvate (PHP) concomitant with the reduction of NAD+.  PGDH is required for tumor cell proliferation, but extracellular l-serine is not able to support cell proliferation. 
Biochemical Properties catalytic activity is regulated by feedback inhibition by L-serine; human enzyme appears to contain bound cofactor when isolated and the binding order appears to be coenzyme before substrate; slowly convert αKG to αHG. Three types of PGDH can be distinguished based on their domain structure. Type III PGDHs contain only a nucleotide binding and substrate binding domain. Type II PGDHs contain an additional regulatory domain (ACT domain), and Type I PGDHs contain a fourth domain, termed the ASB domain. There is no consistent pattern of domain content that correlates with organism type, and even when additional domains are present, they are not always functional.
Significance in milk synthesise L-serine, an important nutrient for brain function and development
PTMs N6-acetyllysine,Phosphoserine,Isopeptide bond,Ubl conjugation.
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Bibliography 1. Davis, J. L. and Fallon, H. J. (1970) ‘Studies on the role of 3-phosphoglycerate dehydrogenase in the regulation of serine biosynthesis in rat liver.’, The Journal of biological chemistry, 245(21), pp. 5838–46. Available at: http://www.ncbi.nlm.nih.gov/pubmed/5479453 (Accessed: 3 October 2019).
2. NAGAMACHI, S. et al. (2018) ‘Dietary L-serine modifies free amino acid composition of maternal milk and lowers the body weight of the offspring in mice’, Journal of Veterinary Medical Science, 80(2), pp. 235–241. doi: 10.1292/jvms.17-0577. 3.Grant GA. D-3-Phosphoglycerate Dehydrogenase. Front Mol Biosci. 2018 Dec 13;5:110. doi: 10.3389/fmolb.2018.00110. PMID: 30619878; PMCID: PMC6300728.