Primary Information
BoMiProt ID	Bomi336
Protein Name	Target of myb1
Organism	Bos taurus
Uniprot ID	Q5BIP4
Milk Fraction	Exosome
Ref Sequence ID	NP_001030187.1
Aminoacid Length	492
Molecular Weight	54212
FASTA Sequence	Download
Gene Name	TOM1
Gene ID	504912
Protein Existence Status	Unreviewed: Experimental evidence at transcript level
Secondary Information
Protein Function	Novel ubiquitin-binding protein; may function as a signal for recruitment of other ubiquitin-binding proteins
Biochemical Properties	Prefers polyubiquitin chain to monoubiquitin, and the region containing the GAT domain and the C-terminal part of the VHS domain is indispensable for ubiquitin binding; Tom1 has a canonical clathrin box and this box is indispensable for clathrin binding; Tollip, a binding partner of Tom1; interact with polyubiquitin chain; stronger ability to bind tetraubiquitin than smaller ubiquitin chains, including monoubiquitin; Tom1 has the ability to bind the Lys-48-linked polyubiquitin chain, although the Lys-48-linked polyubiquitin chain is thought to function as a signal for proteasome-mediated protein degradation; Tom1 was found to be a new clathrin-binding protein and to have a canonical clathrin box
Significance in milk	Associated with secretory activation of mammary glans
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation
Bibliography	1. Rudolph, M. C. et al. (2003) ‘Functional development of the mammary gland: use of expression profiling and trajectory clustering to reveal changes in gene expression during pregnancy, lactation, and involution.’, Journal of mammary gland biology and neoplasia, 8(3), pp. 287–307. Available at: http://www.ncbi.nlm.nih.gov/pubmed/14973374 (Accessed: 3 October 2019). 2. Yamakami, M., Yoshimori, T. and Yokosawa, H. (2003) ‘Tom1, a VHS Domain-containing Protein, Interacts with Tollip, Ubiquitin, and Clathrin’, Journal of Biological Chemistry, 278(52), pp. 52865–52872. doi: 10.1074/jbc.M306740200.