Search by BoMiProt ID - Bomi335


Primary Information

BoMiProt ID Bomi335
Protein Name 6-phosphogluconate dehydrogenase, decarboxylating
Organism Bos taurus
Uniprot IdQ3ZCI4
Milk FractionWhey, MFGM, Exosome
Ref Sequence Id NP_001137210.1
Amino Acid Lenth 483
Molecular Weight 53077
Fasta Sequence https://www.uniprot.org/uniprot/Q3ZCI4.fasta
Gene Name PGD
Gene Id 514939
Protein Existence Status Unreviewed: Experimental evidence at transcript level

Secondry Information

Protein Function enzyme of the pentose phosphate pathway, providing the necessary compounds of NADPH for the synthesis of fatty acids; oxidative carboxylase that catalyzes the decarboxylating reduction of 6- phosphogluconate into ribulose 5-phosphate in the presence of NADP; functional importance of the enzyme is in providing NADPH for fat synthesis and ribose for nucleic acid synthesis;
Biochemical Properties The enzyme follows a rapid equilibrium bi-ter sequential mechanism, with a random order of substrate addition both in the direct reaction, oxidative decarboxylation, and, in the reverse reaction, reductive carboxylation; substrate of reductive carboxylation is ribulose-5-phosphate; 6PG at low concentrations decreases the Km of Ru5P; 6PG is like an allosteric activator of 6PGDH
Significance in milk As found in ovine, 6PGD activity in tail adipose tissue is reduced with the increase of milk energy; In ewes with high milk production, the pathway of lipogenesis is limited by reducing the size of fat cells and reducing the activity of 6PGD
Bibliography 1. Hanau, S. et al. (2010) ‘6-Phosphogluconate Dehydrogenase Mechanism’, Journal of Biological Chemistry, 285(28), pp. 21366–21371. doi: 10.1074/jbc.M110.105601.
2. Trivizaki, S. et al. (2010) ‘Enzymatic and mRNA Transcript Response of Ovine 6-Phosphogluconate Dehydrogenase (6PGD) in Respect to Different Milk Yield.’, Biochemistry research international. Hindawi, 2010, p. 512056. doi: 10.1155/2010/512056.