Search by BoMiProt ID - Bomi335

Primary Information

BoMiProt ID Bomi335
Protein Name 6-phosphogluconate dehydrogenase, decarboxylating
Organism Bos taurus
Uniprot IdQ3ZCI4
Milk FractionWhey, MFGM, Exosome
Ref Sequence Id NP_001137210.1
Aminoacid Length 483
Molecular Weight 53077
Fasta Sequence
Gene Name PGD
Gene Id 514939
Protein Existence Status Unreviewed: Experimental evidence at transcript level

Secondary Information

Protein Function enzyme of the pentose phosphate pathway, providing the necessary compounds of NADPH for the synthesis of fatty acids; oxidative carboxylase that catalyzes the decarboxylating reduction of 6- phosphogluconate into ribulose 5-phosphate in the presence of NADP; functional importance of the enzyme is in providing NADPH for fat synthesis and ribose for nucleic acid synthesis;
Biochemical Properties The enzyme follows a rapid equilibrium bi-ter sequential mechanism, with a random order of substrate addition both in the direct reaction, oxidative decarboxylation, and, in the reverse reaction, reductive carboxylation; substrate of reductive carboxylation is ribulose-5-phosphate; 6PG at low concentrations decreases the Km of Ru5P; 6PG is like an allosteric activator of 6PGDH
Significance in milk As found in ovine, 6PGD activity in tail adipose tissue is reduced with the increase of milk energy; In ewes with high milk production, the pathway of lipogenesis is limited by reducing the size of fat cells and reducing the activity of 6PGD
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Bibliography 1. Hanau, S. et al. (2010) ‘6-Phosphogluconate Dehydrogenase Mechanism’, Journal of Biological Chemistry, 285(28), pp. 21366–21371. doi: 10.1074/jbc.M110.105601.
2. Trivizaki, S. et al. (2010) ‘Enzymatic and mRNA Transcript Response of Ovine 6-Phosphogluconate Dehydrogenase (6PGD) in Respect to Different Milk Yield.’, Biochemistry research international. Hindawi, 2010, p. 512056. doi: 10.1155/2010/512056.