Search by BoMiProt ID - Bomi320


Primary Information

BoMiProt ID Bomi320
Protein Name Adenylosuccinate lyase
Organism Bos taurus
Uniprot IDA3KN12
Milk FractionExosome
Ref Sequence ID NP_001095847.1
Aminoacid Length 490
Molecular Weight 55484
FASTA Sequence Download
Gene Name ADSL
Gene ID 510949
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function bifunctional enzyme acting in de novo purine synthesis and purine nucleotide recycling; catalyses the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) into aminoimidazole carboxamide ribotide (AICAR) in the purine de novo synthesis pathway, and the formation of adenosine monophosphate (AMP) from adenylosuccinate (S-AMP) in the purine nucleotide cycle
Biochemical Properties at several temperatures below 53°C wild-type Thyredoxin–ADSL and several mutants were stable for at least 60 min; N-terminal-truncated ADSL, has thermolability of the wild-type enzyme above 35°C; catalytic base (His141) and the catalytic acid (His68), Gln212 and Asn270 might play a vital role in catalysis by properly orienting the succinyl moiety of the substrates
PTMs N-acetylation at Alanine,Isopeptide bond formation,Ubl Conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Adenylosuccinate lyase hydroxylation contributes to triple negative breast cancer via the activation of cMYC
Bibliography 1. Toth, E. A. and Yeates, T. O. (2000) ‘The structure of adenylosuccinate lyase, an enzyme with dual activity in the de novo purine biosynthetic pathway.’, Structure (London, England : 1993), 8(2), pp. 163–74. doi: 10.1016/s0969-2126(00)00092-7.
2. Kmoch, S. et al. (2000) ‘Human adenylosuccinate lyase (ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients’, Human Molecular Genetics, 9(10), pp. 1501–1513. doi: 10.1093/hmg/9.10.1501.
3. Race, V. et al. (2000) ‘Clinical, biochemical and molecular genetic correlations in adenylosuccinate lyase deficiency’, Human Molecular Genetics, 9(14), pp. 2159–2165. doi: 10.1093/hmg/9.14.2159.