Primary Information |
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BoMiProt ID | Bomi320 |
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Protein Name | Adenylosuccinate lyase |
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Organism | Bos taurus |
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Uniprot ID | A3KN12 |
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Milk Fraction | Exosome |
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Ref Sequence ID | NP_001095847.1 |
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Aminoacid Length | 490 |
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Molecular Weight | 55484 |
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FASTA Sequence |
Download |
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Gene Name | ADSL |
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Gene ID | 510949 |
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Protein Existence Status | Reviewed: Experimental evidence at transcript level |
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Secondary Information |
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Protein Function | bifunctional
enzyme acting in de novo purine synthesis and purine
nucleotide recycling; catalyses the conversion of succinylaminoimidazole
carboxamide ribotide (SAICAR) into
aminoimidazole carboxamide ribotide (AICAR) in the purine de
novo synthesis pathway, and the formation of adenosine monophosphate
(AMP) from adenylosuccinate (S-AMP) in the purine
nucleotide cycle |
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Biochemical Properties | at several temperatures below
53°C wild-type Thyredoxin–ADSL and several mutants were stable for
at least 60 min; N-terminal-truncated ADSL, has thermolability of the wild-type enzyme above
35°C; catalytic base (His141) and the catalytic acid (His68), Gln212
and Asn270 might play a vital role in catalysis by properly orienting the succinyl
moiety of the substrates |
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PTMs | N-acetylation at Alanine,Isopeptide bond formation,Ubl Conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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Predicted Disorder Regions | NA |
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DisProt Annotation | |
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TM Helix Prediction | No TM helices |
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Significance of PTMs | Adenylosuccinate lyase hydroxylation contributes to triple negative breast cancer via the activation of cMYC |
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Bibliography | 1. Toth, E. A. and Yeates, T. O. (2000) ‘The structure of adenylosuccinate lyase, an enzyme with dual activity in the de novo purine biosynthetic pathway.’, Structure (London, England : 1993), 8(2), pp. 163–74. doi: 10.1016/s0969-2126(00)00092-7. 2. Kmoch, S. et al. (2000) ‘Human adenylosuccinate lyase (ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients’, Human Molecular Genetics, 9(10), pp. 1501–1513. doi: 10.1093/hmg/9.10.1501. 3. Race, V. et al. (2000) ‘Clinical, biochemical and molecular genetic correlations in adenylosuccinate lyase deficiency’, Human Molecular Genetics, 9(14), pp. 2159–2165. doi: 10.1093/hmg/9.14.2159. |