Primary Information
BoMiProt ID	Bomi314
Protein Name	14 kDa phosphohistidine phosphatase
Organism	Bos taurus
Uniprot ID	Q32PA4
Milk Fraction	Exosome
Ref Sequence ID	NP_001069984.1
Aminoacid Length	125
Molecular Weight	13931
FASTA Sequence	Download
Gene Name	PHPT1
Gene ID	618691
Protein Existence Status	Reviewed: Experimental evidence at transcript level
Secondary Information
Presence in other biological fluids/tissue/cells	highly expressed in hepatocellular carcinoma (HCC) tissues and cell lines compared with adjacent non‑cancerous human liver tissues and cells
Protein Function	14-kDa Phosphohistidine phosphatase (PHP14), the first histidine phosphatase protein identified in vertebrates, was recently revealed to play an essential role in lung cancer.dephosphorylates proteins and peptides that are phosphorylated on a histidine residue; ion transportation was activated by the phosphorylation and deactivated by a PHPT1-dependent dephosphorylation; possible role in cytoskeletal reorganization and in hepatocellular carcinoma cell proliferation; dephosphorylate ATP‑citrate lyase and the β‑subunit of G proteins in vitro; may play a role in neuronal signaling transduction
Biochemical Properties	No activity of this enzyme could be detected toward a set of O-phosphorylated peptides containing phosphoserine, phosphothreonine, or phosphotyrosine; activity was independent of divalent cations, and okadaic acid; His-53 was shown to be essential for the phosphatase activity toward phosphohistidine
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	Inhibition of PHP14 decreased the expression of the fibrogenic signature at the early stage of liver fibrogenesis and the activation of HSCs in vivo. Thus, PHP14 can be considered a potential therapeutic target for liver fibrosis.
Bibliography	1. Han, S.-X. et al. (2012) ‘14-kDa Phosphohistidine phosphatase plays an important role in hepatocellular carcinoma cell proliferation.’, Oncology letters, 4(4), pp. 658–664. doi: 10.3892/ol.2012.802. 2. Ek, P., Ek, B. and Zetterqvist, Ö. (2015) ‘Phosphohistidine phosphatase 1 (PHPT1) also dephosphorylates phospholysine of chemically phosphorylated histone H1 and polylysine’, Upsala Journal of Medical Sciences, 120(1), pp. 20–27. doi: 10.3109/03009734.2014.996720. 2.Han SX, Wang LJ, Zhao J, Zhang Y, Li M, Zhou X, Wang J, Zhu Q. 14-kDa Phosphohistidine phosphatase plays an important role in hepatocellular carcinoma cell proliferation. Oncol Lett. 2012 Oct;4(4):658-664. doi: 10.3892/ol.2012.802. Epub 2012 Jul 13. PMID: 23205079; PMCID: PMC3506665. 3.Xu A, Zhou J, Li Y, Qiao L, Jin C, Chen W, Sun L, Wu S, Li X, Zhou D, Jia S, Zhang B, Yao J, Zhang X, You H, Huang J. 14-kDa phosphohistidine phosphatase is a potential therapeutic target for liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 2021 Mar 1;320(3):G351-G365. doi: 10.1152/ajpgi.00334.2020. Epub 2021 Jan 6. PMID: 33406007.