Search by BoMiProt ID - Bomi294


Primary Information

BoMiProt ID Bomi294
Protein Name Integrin-linked protein kinase
Organism Bos taurus
Uniprot IdQ3SWY2
Milk FractionExosome
Ref Sequence Id NP_001029865.1
Amino Acid Lenth 452
Molecular Weight 51447
Fasta Sequence https://www.uniprot.org/uniprot/Q3SWY2.fasta
Gene Name ILK
Gene Id 540207
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondry Information

Protein Function involved in diverse cell adhesion-dependent physiological and pathological responses; binding partner of integrin cytoplasmic tails and was found to critically regulate fatty acids; can act as a scaffold protein to function through cellematrix interactions, cell signaling, and cytoskeletal organization; mediates many important cellular processes, including survival, proliferation, differentiation, adhesion, migration, contractility; plays some role in the activation of endothelial progenitor cells and neovascularization, and may also enhance vascular endothelial growth factor expression. Increased ILK activity may promote epithelial-to-mesenchymal transition and induce a transformed, tumorigenic phenotype
Biochemical Properties ILK has kinase activity and a preference for manganese when phosphorylating GSK-3; ILK was capable of directly phosphorylating diverse substrates including a generic substrate myelin basic protein and physiological targets including integrin ß 1 cytoplasmic tails, myosin light chain kinase LC20, cell survival kinase AKT/PKB, and glycogen synthase kinase-3 (GSK-3 ); localizes to focal adhesions, and interacts with the cytoplasmic tail of b subunits of integrins and couples them to the actin cytoskeleton
Significance in milk Forced expression of a dominant negative, kinase-dead form of ILK subtly altered mouse mammary epithelial cell morphogenesis but it did not prevent differentiative milk protein expression
Bibliography 1. Fukuda, K. et al. (2011) ‘Biochemical, Proteomic, Structural, and Thermodynamic Characterizations of Integrin-linked Kinase (ILK)’, Journal of Biological Chemistry, 286(24), pp. 21886–21895. doi: 10.1074/jbc.M111.240093.
2. Somasiri, A. et al. (2001) ‘Overexpression of the integrin-linked kinase mesenchymally transforms mammary epithelial cells.’, Journal of cell science, 114(Pt 6), pp. 1125–36. Available at: http://www.ncbi.nlm.nih.gov/pubmed/11228156 (Accessed: 4 October 2019).