Search by BoMiProt ID - Bomi284

Primary Information

BoMiProt ID Bomi284
Protein Name Deoxyribonuclease (Fragment)
Organism Bos taurus
Uniprot IdF1MW13
Milk FractionMFGM, Exosome
Amino Acid Lenth 316
Molecular Weight 35174
Fasta Sequence
Gene Name DNASE1L1
Protein Existence Status Unreviewed: Protein inferred from homology

Secondry Information

Presence in other biological fluids/tissue/cells Pancreas, Kidney ,Liver ,Spleen ,Heart ,Lung ,Stomach ,Small intestine,Cerebrum ,Cerebellum ,Parotid gland ,Submaxillary gland ,Testis ,Epididymis
Protein Function perform a variety of important cellular roles by degrading DNA via hydrolysis of its phosphodiester backbone; cleave single or double-stranded DNA and require divalent metal ions to hydrolyze DNA yielding 3 -hydroxyl and 5 -phosphorylated products
Biochemical Properties divalent cation-dependent endonuclease originally isolated from bovine pancreas; G-actin is known to be a potent inhibitor of mammalian DNase I -the activity of mouse DNase I was inhibited as readily as that of human DNase I by G-actin; all DNase I family proteins contain hydrophobic precursor peptides in their N-termini, two essential histidine residues of DNase I are conserved in the other members, all are activated by Ca2+ and Mg2+ and are strongly inhibited by Zn2+, and members hydrolyse DNA endonucleolytically to produce 3' -OH/5' -P ends; DNase X has an extra hydrophobic stretch in its C-terminus; optimal activity at the low pH environment of lysosomes
Significance in milk protect against viruses in milk owing to the ability to inhibit the activity of RNAdependent DNA-polymerase
PTMs Glycosylated: In humans, N-linked glycosylation at Asn-243- conserved in the corresponding regions of monkey, porcine and bovine DNase X proteins
Bibliography 1. Takeshita, H. et al. (1997) ‘Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution.’, Biochemistry and molecular biology international, 42(1), pp. 65–75. Available at: (Accessed: 4 October 2019).
2. Shiokawa, D. et al. (2005) ‘Physical and biochemical properties of mammalian DNase X proteins: non-AUG translation initiation of porcine and bovine mRNAs for DNase X’, Biochemical Journal, 392(3), pp. 511–517. doi: 10.1042/BJ20051114.
3. Varela-Ramirez, A. et al. (2017) ‘Structure of acid deoxyribonuclease’, Nucleic Acids Research, 45(10), pp. 6217–6227. doi: 10.1093/nar/gkx222.