Primary Information |
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BoMiProt ID | Bomi2765 |
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Protein Name | Histidyl-tRNA synthetase, cytoplasmic |
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Organism | Bos taurus |
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Uniprot ID | Q2KI84 |
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Milk Fraction | Exosome |
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Ref Sequence ID | NP_001039531.1 |
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Aminoacid Length | 509 |
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Molecular Weight | 57285 |
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FASTA Sequence |
Download |
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Gene Name | HARS |
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Gene ID | 510937 |
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Protein Existence Status | Reviewed: Experimental evidence at transcript level |
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Secondary Information |
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Protein Function | Histidyl-tRNA synthetase (HisRS) is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. |
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Biochemical Properties | Primary structures of HisRS shows that the subunits of these homo-dimeric enzymes consist of 420-550 amino acid residues. HisRS from Escherichia coli and Thermus thermophilus are very similar dimeric enzymes consisting of three domains: the N-terminal catalytic domain containing the six-stranded antiparallel beta-sheet and the three motifs characteristic of class II aaRS, a HisRS-specific helical domain inserted between motifs 2 and 3 that may contact the acceptor stem of the tRNA, and a C-terminal alpha/beta domain that may be involved in the recognition of the anticodon stem and loop of tRNA(His). |
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PTMs | Phosphorylation at Serine,N-acetylation at Alanine |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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Predicted Disorder Regions | (344-355) |
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DisProt Annotation | |
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TM Helix Prediction | No TM helices |
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Bibliography | Freist W, Verhey JF, Rühlmann A, Gauss DH, Arnez JG. Histidyl-tRNA synthetase. Biol Chem. 1999 Jun;380(6):623-46. doi: 10.1515/BC.1999.079. PMID: 10430027. |