Search by BoMiProt ID - Bomi276


Primary Information

BoMiProt ID Bomi276
Protein Name Dihydrofolate reductase
Organism Bos taurus
Uniprot IDP00376
Milk FractionMFGM, Exosome
Ref Sequence ID NP_001071351.1
Aminoacid Length 187
Molecular Weight 21604
FASTA Sequence Download
Gene Name DHFR
Gene ID 508809
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondary Information

Protein Function catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, which is in turn converted to a key cofactor required for several one-carbon transfer reactions that are critical for the biosynthesis of DNA, RNA and certain amino acids; DHFR inhibitors are expected to be good alternatives to fight bacterial infections.
Biochemical Properties monomeric enzyme consists of an eight-stranded beta sheet structure with seven parallel strands and five helices that fold against the active site core; amino acid sequence of bovine DHFR is highly homologous to other mammalian enzymes, with that of murine DHFR as the closest homologue; Comparison of amino acid sequences revealed 19 substitutions between murine and bovine DHFR with the majority of these changes observed outside of the active site; two forms of bDHFR in a ratio of 20:1, with pIs of 7.15 and 5.94 and identical specific activities with dihydrofolate and folate
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Bibliography 1. Cody, V., Mao, Q. and Queener, S. F. (2008) ‘Recombinant bovine dihydrofolate reductase produced by mutagenesis and nested PCR of murine dihydrofolate reductase cDNA.’, Protein expression and purification, 62(1), pp. 104–10. doi: 10.1016/j.pep.2008.07.001.
2. Lai, P. H. et al. (1982) ‘Structure of dihydrofolate reductase: primary sequence of the bovine liver enzyme.’, Biochemistry, 21(14), pp. 3284–94. doi: 10.1021/bi00257a006. 3.He J, Qiao W, An Q, Yang T, Luo Y. Dihydrofolate reductase inhibitors for use as antimicrobial agents. Eur J Med Chem. 2020 Jun 1;195:112268. doi: 10.1016/j.ejmech.2020.112268. Epub 2020 Apr 1. PMID: 32298876. 4.Gangjee A, Kurup S, Namjoshi O. Dihydrofolate reductase as a target for chemotherapy in parasites. Curr Pharm Des. 2007;13(6):609-39. doi: 10.2174/138161207780162827. PMID: 17346178.