Search by BoMiProt ID - Bomi2672


Primary Information

BoMiProt ID Bomi2672
Protein Name GTP-binding nuclear protein Ran
Organism Bos taurus
Uniprot IDQ3T054
Milk FractionExosome
Ref Sequence ID NP_001029877.1
Aminoacid Length 216
Molecular Weight 24423
FASTA Sequence Download
Gene Name RAN
Gene ID 540457
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function Ran is a small GTP-binding protein of the Ras superfamily regulating fundamental cellular processes: nucleo-cytoplasmic transport, nuclear envelope formation and mitotic spindle assembly.
Biochemical Properties As a member of the Ras superfamily, Ran is structurally composed of a fold known as the G-domain (GTP-binding domain), a central six-stranded β-sheet that is surrounded by α-helices. Ras-family members bind to GTP and GDP nucleotides with high picomolar affinity. However, only in the GTP-bound form and the switch I- and switch II-loops adopt a stable conformation.
PTMs N6-Acetylation at Lys, Isopeptide bond formation, Phosphorylation at Thr, Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions 33-44,123-144,170-216
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Acetylation at Lys residues interferes with essential aspects of Ran function: nucleotide exchange and hydrolysis, subcellular Ran localization, GTP hydrolysis, and the interaction with import and export receptors.Acetylation by KAT5 at Lys-134 is increased during mitosis, impairs RANGRF binding and enhances RCC1 binding.
Bibliography 1.de Boor S, Knyphausen P, Kuhlmann N, Wroblowski S, Brenig J, Scislowski L, Baldus L, Nolte H, Krüger M, Lammers M. Small GTP-binding protein Ran is regulated by posttranslational lysine acetylation. Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):E3679-88. doi: 10.1073/pnas.1505995112. Epub 2015 Jun 29. PMID: 26124124; PMCID: PMC4507232.