Search by BoMiProt ID - Bomi2534


Primary Information

BoMiProt ID Bomi2534
Protein Name Receptor-type tyrosine-protein phosphatase F
Organism Bos taurus
Uniprot IDA7MBJ4
Milk FractionExosome
Ref Sequence ID NP_001094549.1
Aminoacid Length 1898
Molecular Weight 211382
FASTA Sequence Download
Gene Name PTPRF
Gene ID 512072
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function PTPRF, a receptor-type tyrosine phosphatase, in regulating Wnt signaling in CRC.
Biochemical Properties PTPRF consists of an extracellular domain (E-domain), a single transmembrane domain (TM) and an intracellular phosphatase domain (P-domain).The E-domain of PTPRF contains three immunoglobulin (Ig)-like and eight fibronectin type-III motifs, and a post-translational proteolytic cleavage separates the E-domain but it remains attached to the TM domain and the rest of the protein. The P-domain of PTPRF is comprised of a catalytically active D1 domain and a pseudophosphatase D2 domain.
PTMs Disulfide bond formation,N-Linked Glycosylation at Asn, Phosphorylation at Ser
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions 399-418, 693-713
DisProt Annotation
TM Helix Prediction 1TMH; (1253-1275)
Additional Comments Knockdown of PTPRF decreased cell proliferation in patient-derived primary colon cancer cells and established CRC cell lines. 
Bibliography 1.Gan T, Stevens AT, Xiong X, Wen YA, Farmer TN, Li AT, Stevens PD, Golshani S, Weiss HL, Evers BM, Gao T. Inhibition of protein tyrosine phosphatase receptor type F suppresses Wnt signaling in colorectal cancer. Oncogene. 2020 Oct;39(44):6789-6801. doi: 10.1038/s41388-020-01472-z. Epub 2020 Sep 24. PMID: 32973331; PMCID: PMC7606795. 2.Streuli M, Krueger NX, Ariniello PD, Tang M, Munro JM, Blattler WA et al. Expression of the receptor-linked protein tyrosine phosphatase LAR: proteolytic cleavage and shedding of the CAM-like extracellular region. The EMBO journal 1992; 11: 897–907. 3.Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I et al. Large-scale structural analysis of the classical human protein tyrosine phosphatome. Cell 2009; 136: 352–363.