Search by BoMiProt ID - Bomi253


Primary Information

BoMiProt ID Bomi253
Protein Name Collectin-12
Organism Bos taurus
Uniprot IdA6QP79
Milk FractionExosome
Ref Sequence Id NP_001095313.1
Aminoacid Length 742
Molecular Weight 81751
Fasta Sequence https://www.uniprot.org/uniprot/A6QP79.fasta
Gene Name COLEC12
Gene Id 504741
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Presence in other biological fluids/tissue/cells placenta, lung, heart, astrocytes, microglia, vascular/perivascular cells of the brain and in cultured nurse-like cell lines
Protein Function mediate phagocytosis of Saccharomyces,E. coli and S. aureus; Cl-P1 in the vascular spaces acts as a scavenger receptor to scavenge released neutrophil glycoproteins, both locally at sites of inflammation or systemically,when they are released in the circulation; CL-P1 bound CRP in a charge-dependent manner interaction mediated activation of the classical complement activation pathway. Collectin-12 (collectin placenta 1, CL-P1, or CL-12) is a newly identified pattern recognition molecule of the innate immune system. Recent evidences show that CL-12 plays important roles not only in innate immune protection against certain clinically important pathogens but also in scavenging of host molecules, leukocyte recruitment, and cancer metastasis. 
Biochemical Properties single pass (type II) transmembrane protein orientated with its N-terminal towards the cytosol; trimer of polypeptide chains, initiated and stabilized by formation of the α helical coiled-coil and the collagen-like region; regarded as both a collectin and a scavenger receptor; possesses three positive charged clusters in the collagen-like region, which are highly conserved in CL-P1 from various species; does not bind to the majority of Lewis structures that are sialylated
Significance in milk Antimicrobial proteins - exclusively detected in the colostrum fluid phase
PTMs Disulfide bond formation and N-Linked Glycosylation at Asn
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions 439-591
DisProt Annotation
TM Helix Prediction 1TMH; (40-62)
Bibliography 1. Ohtani, K. et al. (2001) ‘The Membrane-type Collectin CL-P1 Is a Scavenger Receptor on Vascular Endothelial Cells’, Journal of Biological Chemistry, 276(47), pp. 44222–44228. doi: 10.1074/jbc.M103942200.
2. Nakamura, K. et al. (2001) ‘Molecular Cloning and Functional Characterization of a Human Scavenger Receptor with C-Type Lectin (SRCL), a Novel Member of a Scavenger Receptor Family’, Biochemical and Biophysical Research Communications, 280(4), pp. 1028–1035. doi: 10.1006/bbrc.2000.4210.
3. Mori, K. et al. (2014) ‘Scavenger receptor CL-P1 mainly utilizes a collagen-like domain to uptake microbes and modified LDL’, Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(12), pp. 3345–3356. doi: 10.1016/j.bbagen.2014.08.015.
4. Yoshida, T. et al. (2003) ‘SRCL/CL-P1 recognizes GalNAc and a carcinoma-associated antigen, Tn antigen.’, Journal of biochemistry, 133(3), pp. 271–7. doi: 10.1093/jb/mvg037.
5. Roy, N. et al. (2016) ‘Collectin CL-P1 utilizes C-reactive protein for complement activation’, Biochimica et Biophysica Acta (BBA) - General Subjects, 1860(6), pp. 1118–1128. doi: 10.1016/j.bbagen.2016.02.012.
6. Jang, S. et al. (2009) ‘Scavenger Receptor Collectin Placenta 1 (CL-P1) Predominantly Mediates Zymosan Phagocytosis by Human Vascular Endothelial Cells’, Journal of Biological Chemistry, 284(6), pp. 3956–3965. doi: 10.1074/jbc.M807477200.
7. Ohtani, K. et al. (1999) ‘Molecular Cloning of a Novel Human Collectin from Liver (CL-L1)’, Journal of Biological Chemistry, 274(19), pp. 13681–13689. doi: 10.1074/jbc.274.19.13681. 8.Zhang J, Li A, Yang CQ, Garred P, Ma YJ. Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens. J Immunol Res. 2019 Jul 29;2019:9164202. doi: 10.1155/2019/9164202. PMID: 31482100; PMCID: PMC6701420.