Primary Information |
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BoMiProt ID | Bomi2483 |
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Protein Name | Prohibitin-2 |
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Organism | Bos taurus |
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Uniprot ID | Q2HJ97 |
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Milk Fraction | MFGM |
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Ref Sequence ID | NP_001039663.1 |
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Aminoacid Length | 299 |
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Molecular Weight | 33357 |
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FASTA Sequence |
Download |
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Gene Name | PHB2 |
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Gene ID | 515363 |
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Protein Existence Status | Reveiwed:Experimental evidence at transcript level |
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Secondary Information |
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Protein Function | Prohibitin 2 (PHB2), as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation. PHB2 binds the autophagosomal membrane-associated protein LC3 through an LC3-interaction region (LIR) domain upon mitochondrial depolarization and proteasome-dependent outer membrane rupture. |
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Biochemical Properties | There are three candidate LIR domains (W/F/YxxL/I/V motifs) in PHB2, including one that is unique to PHB2 (amino acids [aa] 77–80) and two that are conserved between PHB1 and PHB2 (aa 121–124 and aa 175–178). |
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PTMs | N-Acetylation at Ala, Phosphorylation at Tyr by IGFBP6 |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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Predicted Disorder Regions | NA |
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DisProt Annotation | |
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TM Helix Prediction | No TM helices |
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Additional Comments | A mutation in the other candidate LIR domain, F175A/I178A, led to lower levels of protein expression but did not abrogate binding to GST-LC3. |
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Bibliography | Wei Y, Chiang WC, Sumpter R Jr, Mishra P, Levine B. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor. Cell. 2017 Jan 12;168(1-2):224-238.e10. doi: 10.1016/j.cell.2016.11.042. Epub 2016 Dec 22. PMID: 28017329; PMCID: PMC5235968. |