Search by BoMiProt ID - Bomi2483


Primary Information

BoMiProt ID Bomi2483
Protein Name Prohibitin-2
Organism Bos taurus
Uniprot IDQ2HJ97
Milk FractionMFGM
Ref Sequence ID NP_001039663.1
Aminoacid Length 299
Molecular Weight 33357
FASTA Sequence Download
Gene Name PHB2
Gene ID 515363
Protein Existence Status Reveiwed:Experimental evidence at transcript level

Secondary Information

Protein Function Prohibitin 2 (PHB2), as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation. PHB2 binds the autophagosomal membrane-associated protein LC3 through an LC3-interaction region (LIR) domain upon mitochondrial depolarization and proteasome-dependent outer membrane rupture. 
Biochemical Properties There are three candidate LIR domains (W/F/YxxL/I/V motifs) in PHB2, including one that is unique to PHB2 (amino acids [aa] 77–80) and two that are conserved between PHB1 and PHB2 (aa 121–124 and aa 175–178).
PTMs N-Acetylation at Ala, Phosphorylation at Tyr by IGFBP6
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments A mutation in the other candidate LIR domain, F175A/I178A, led to lower levels of protein expression but did not abrogate binding to GST-LC3.
Bibliography Wei Y, Chiang WC, Sumpter R Jr, Mishra P, Levine B. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor. Cell. 2017 Jan 12;168(1-2):224-238.e10. doi: 10.1016/j.cell.2016.11.042. Epub 2016 Dec 22. PMID: 28017329; PMCID: PMC5235968.