Primary Information
BoMiProt ID	Bomi2446
Protein Name	Nucleobindin-1
Organism	Bos taurus
Uniprot ID	Q0P569
Milk Fraction	Whey, Exosome
Ref Sequence ID	NP_001068667.1
Aminoacid Length	474
Molecular Weight	54982
FASTA Sequence	Download
Gene Name	NUCB1
Gene ID	505351
Protein Existence Status	Reviewed: Experimental evidence at transcript level
Secondary Information
Protein Function	The cis-Golgi protein nucleobindin-1 (NUCB1) is critical for MMP2(Matrix metalloproteinases)and MT1-MMP trafficking along the Golgi apparatus.
Biochemical Properties	NUCB1 and Cab45 share similar features, such as the luminal exposure of their Ca2+ binding Efhs.NUCB1 EFh motifs have an open conformation under physiological conditions and fold upon Ca2+ addition.
PTMs	Phosphorylation at Ser/Thr
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation
Predicted Disorder Regions	190-218,382-474
DisProt Annotation
TM Helix Prediction	No TM helices
Bibliography	1.Pacheco-Fernandez N, Pakdel M, Blank B, Sanchez-Gonzalez I, Weber K, Tran ML, Hecht TK, Gautsch R, Beck G, Perez F, Hausser A, Linder S, von Blume J. Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs. J Cell Biol. 2020 Aug 3;219(8):e201907058. doi: 10.1083/jcb.201907058. PMID: 32479594; PMCID: PMC7401813. 2.de Alba, E., and N. Tjandra. 2004. Structural studies on the Ca2+ binding domain of human nucleobindin (calnuc). Biochemistry. 43:10039–10049.https://doi.org/10.1021/bi049310a. 3.Scherer, P.E., G.Z. Lederkremer, S. Williams, M. Fogliano, G. Baldini, and H.F.Lodish. 1996. Cab45, a novel (Ca2+)-binding protein localized to the Golgi lumen. J. Cell Biol. 133:257–268. https://doi.org/10.1083/jcb.133.2.257. 4.von Blume, J., A.-M. Alleaume, C. Kienzle, A. Carreras-Sureda, M. Valverde and V. Malhotra. 2012. Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network. J. Cell Biol. 199:1057–1066.https://doi.org/10.1083/jcb.201207180.