Primary Information |
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| BoMiProt ID | Bomi2336 |
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| Protein Name | Eukaryotic initiation factor 4A-I |
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| Organism | Bos taurus |
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| Uniprot ID | Q3SZ54 |
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| Milk Fraction | MFGM |
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| Ref Sequence ID | NP_001029400.1 |
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| Aminoacid Length | 406 |
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| Molecular Weight | 46154 |
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| FASTA Sequence |
Download |
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| Gene Name | EIF4A1 |
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| Gene ID | 504958 |
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| Protein Existence Status | Reviewed: Experimental evidence at transcript level |
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Secondary Information |
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| Protein Function | eIF-4A is a translation initiation factor that exhibits bidirectional RNA unwinding activity in vitro in the presence of another translation initiation factor, eIF-4B and ATP. |
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| Biochemical Properties | eIF-4A is a member of a fast growing family of proteins termed the DEAD family. These proteins are believed to be RNA helicases, based on the demonstrated in vitro RNA helicase activity of two members (eIF-4A and p68) and their homology in eight amino acid regions. Several related biochemical activities were attributed to eIF-4A: (i) ATP binding, (ii) RNA-dependent ATPase and (iii) RNA helicase. |
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| PTMs | N-6 Acetylation at Lys/Ser, Isopeptide bond formation, Phosphorylation at Ser/Thr, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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| Predicted Disorder Regions | (1-21) |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Mutations in the ATPase A motif (AXXXXGKT) affect ATP binding, whereas mutations in the predicted ATPase B motif (DEAD) affect ATP hydrolysis. |
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| Bibliography | Pause A, Sonenberg N. Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A. EMBO J. 1992 Jul;11(7):2643-54. PMID: 1378397; PMCID: PMC556740. |
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