Primary Information | |
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BoMiProt ID | Bomi232 |
Protein Name | Beta-1,4-galactosyltransferase 1 |
Organism | Bos taurus |
Uniprot ID | P08037 |
Milk Fraction | Whey, MFGM, Exosome |
Ref Sequence ID | NP_803478.1 |
Aminoacid Length | 402 |
Molecular Weight | 44843 |
FASTA Sequence | Download |
Gene Name | B4GALT1 |
Gene ID | 281781 |
Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
Protein Function | synthesizes a disaccharide unit, Galβ1-4GlcNAc (N-acetyllactosamine), present in many biologically active carbohydrate determinants, mediating several biological events, making it a key enzyme in glycobiology; participates in the synthesis of Galβ1-4-GlcNacdisaccharide unit of glycoconjugates. Lactosylceramide and paragloboside were synthesized from their precursor glycolipids and UDP-galactose by lactose synthase A protein. |
Biochemical Properties | trans-Golgi glycosyltransferase (Glyco-T) with a type II membrane protein topology, a short N-terminal cytoplasmic domain, a membrane-spanning region, as well as a stem and a C-terminal catalytic domain facing the trans-Golgi-lumen; hydrophobic membrane-spanning region, like that of other Glyco-T, has a shorter length compared to plasma membrane proteins, an important feature for its retention in the trans-Golgi; |
Significance in milk | interacts with α-lactalbumin to form the lactose synthase (LS) complex that transfers galactose from UDP-α-D-Gal to glucose, producing the lactose secreted in milk |
PTMs | Disulfide bond formation and Glycosylation at Asn |
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P08037|B4GT1_BOVIN Beta-1,4-galactosyltransferase 1 OS=Bos taurus OX=9913 GN=B4GALT1 PE=1 SV=3 MKFREPLLGGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLRRLPQLVGVHPPL QGSSHGAAAIGQPSGELRLRGVAPPPPLQN*90SSKPRSRAPSNLDAYSHPGPGPGPGSNLTS APVPSTTTRSLTACPEESPLLVGPMLIEFNIPVDLKLVEQQNPKVKLGGRYTPMDCISPH KVAIIIPFRNRQEHLKYWLYYLHPILQRQQLDYGIYVINQAGESMFNRAKLLNVGFKEAL KDYDYNCFVFSDVDLIPMNDHNTYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQ FLSINGFPNNYWGWGGEDDDIYNRLAFRGMSVSRPNAVIGKCRMIRHSRDKKNEPNPQRF DRIAHTKETMLSDGLNSLTYMVLEVQRYPLYTKITVDIGTPS |
SCOP | Class : Alpha and beta proteins (a/b) Fold : NDP Glycotransferase-like Superfamily : Nucleotide-diphospho-sugar transferases Family : beta 1,4 galactosyltransferase (b4GalT1) Domain Name : 1NMM B:131-402 |
CATH | Matched CATH superfamily 3.90.550.10 |
Predicted Disorder Regions | (58-136) |
DisProt Annotation | |
TM Helix Prediction | 1TMH; (25-43) |
PDB ID | 1FGX, 1FR8, 1NF5, 1NHE, 1NKH, 1NMM, 1NQI, 1NWG, 1O0R, 1O23, 1OQM, 1PZT, 1PZY, 1TVY, 1TW1, 1TW5, 1YRO, 2FYC, 2FYD, 4KRV, |
Bibliography | 1. Qasba, P. K., Ramakrishnan, B. and Boeggeman, E. (2005) ‘Substrate-induced conformational changes in glycosyltransferases’, Trends in Biochemical Sciences, 30(1), pp. 53–62. doi: 10.1016/j.tibs.2004.11.005. 2. Qasba, P., Ramakrishnan, B. and Boeggeman, E. (2008) ‘Structure and Function of β -1,4-Galactosyltransferase’, Current Drug Targets, 9(4), pp. 292–309. doi: 10.2174/138945008783954943. 3. Harrus, D. et al. (2018) ‘The dimeric structure of wild-type human glycosyltransferase B4GalT1.’, PloS one. Edited by I. André, 13(10), p. e0205571. doi: 10.1371/journal.pone.0205571. 4.Yamato K, Yoshida A. Biosynthesis of lactosylceramide and paragloboside by human lactose synthase A protein. J Biochem. 1982 Oct;92(4):1123-7. doi: 10.1093/oxfordjournals.jbchem.a134028. PMID: 6816790. |