Search by BoMiProt ID - Bomi215


Primary Information

BoMiProt ID Bomi215
Protein Name Annexin A2
Organism Bos taurus
Uniprot IdP04272
Milk FractionMFGM, Exosome
Ref Sequence Id NP_777141.1
Amino Acid Lenth 339
Molecular Weight 38612
Fasta Sequence https://www.uniprot.org/uniprot/P04272.fasta
Gene Name ANXA2
Gene Id 282689
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondry Information

Protein Function member of the annexin family of Ca2+ dependent phospholipid binding proteins; AnxA2 significantly increases the phosphorylation of PI3K; regulates autophagy or cell resistance through the Akt/mTOR signaling pathway
Biochemical Properties After phosphorylation AnxA2 can be cleaved by mild chymotrypsin into a 33 kD C terminal core domain and 3 kD N terminal domain of 30 amino acids; Asparaginyl endopeptidase can cleave an N terminal peptide of bovine AnxA2 at a position between Asn31 and Phe32;
Significance in milk AnxA2 might response to amino acids and hormones to activate the mTOR pathway leading to milk synthesis and proliferation of bovine mammary epithelial cells
PTMs N terminus of AnxA2 contains several major sites for PTMs , including the phosphorylation sites Ser11, Ser25 and Tyr23; human placenta annexin A2 contains potential N-linked glycosylation site at the 61th residue of its amino terminal region
Significance of PTMs regulate the localisation and function of the multifunctional protein Annexin A2; Phosphorylation of Ser25 increases the accessibility of the mRNA- and G-actin-binding sites of AnxA2; Ser25 phosphorylated AnxA2 has been implicated in exocytosis, macro-pinosome motility, recycling of lipid rafts and the recruitment of protein kinase C (PKC) to phosphoinositide-4,5-biphosphate-rich membrane domains;
PDB ID 4X9P,
Bibliography 1. Aukrust, I., Rosenberg, L. A., Ankerud, M. M., Bertelsen, V., Hollås, H., Saraste, J., … Vedeler, A. (2017). Post-translational modifications of Annexin A2 are linked to its association with perinuclear nonpolysomal mRNP complexes. FEBS Open Bio, 7(2), 160–173. https://doi.org/10.1002/2211-5463.12173.
2. Zhang, M., Chen, D., Zhen, Z., Ao, J., Yuan, X., & Gao, X. (2018). Annexin A2 positively regulates milk synthesis and proliferation of bovine mammary epithelial cells through the mTOR signaling pathway. Journal of Cellular Physiology, 233(3), 2464–2475. https://doi.org/10.1002/jcp.26123.