Search by BoMiProt ID - Bomi213


Primary Information

BoMiProt ID Bomi213
Protein Name Aldehyde dehydrogenase, mitochondrial
Organism Bos taurus
Uniprot IdP20000
Milk FractionMFGM
Ref Sequence Id NP_001068835.1
Amino Acid Lenth 520
Molecular Weight 56653
Fasta Sequence https://www.uniprot.org/uniprot/P20000.fasta
Gene Name ALDH2
Gene Id 508629
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondry Information

Protein Function represent large family members of NAD(P)+-dependent dehydrogenases responsible for the irreversible metabolism of many endogenous and exogenous aldehydes to the corresponding acids; catalyzes the oxidation of acetaldehyde to acetate during ethanol metabolism;
Biochemical Properties cytosolic and mitochondrial forms, designated as ALDH1 and ALDH2, are both tetrameric with individual subunits comprising 499–500 amino acids and they share 68% sequence identity with each other; vA transition to full-site reactivity, however, occurs at elevated pH or in the presence of Mg2+; low Km enzyme responsible for the metabolism of acetaldehyde and lipid peroxides such as malondialdehyde and 4-hydroxynonenal, both of which are highly reactive and toxic; inhibition of ALDH2 would lead to elevated levels of acetaldehyde and other reactive lipid peroxides; require NAD+ as a cofactor
Significance in milk in lactating rodent mammary epicelial cells, upregulation of aldehyde dehydrogenase which is associated with the breakdown of acetate and beta-hydroxy butyrate to provide additional sources of acetyl CoA, a substrate for the TCA cycle and subsequent ATP synthesis
PTMs cytosolic ALDH1A1, mitochondrial ALDH2, and other isozymes such as ALDH5A1 and ALDH6A1 were oxidatively-modified in rats exposed to alcohol; protein kinases such as protein kinase Cε (PKCε) can phosphorylate and activate mitochondrial ALDH2 - Thr185, Thr412 and possibly Ser279 of ALDH2 could be phosphorylated by PKCε, resulting in ALDH2 activation; ALDH2 could be inhibited through protein nitration based on the recent data where GTN promotes nitration signaling; ALDH2 activity was mildly inhibited by NO or hydrogen peroxide in the experimental conditions, but was markedly inhibited by incubation with peroxynitrite
Significance of PTMs regulates activation and deactivation of the enzyme
PDB ID 1A4Z, 1AG8,
Bibliography 1. Jansen, S., Collins, A., Golden, L., Sokolova, O., & Goode, B. L. (2014). Structure and mechanism of mouse cyclase-associated protein (CAP1) in regulating actin dynamics. Journal of Biological Chemistry, 289(44), 30732–30742. https://doi.org/10.1074/jbc.M114.601765.
2. Yuan, X., Zhen, Z., Zhang, M., Yu, Y., Gao, X., & Ao, J.-X. (2019). Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells. Cell Biochemistry and Function, 37(3), 185–192. https://doi.org/10.1002/cbf.3387.