Search by BoMiProt ID - Bomi208


Primary Information

BoMiProt ID Bomi208
Protein Name Cytoplasmic aconitate hydratase
Organism Bos taurus
Uniprot IDQ0VCU1
Milk FractionMFGM, Exosome
Ref Sequence ID NP_001069059.1
Aminoacid Length 889
Molecular Weight 98204
FASTA Sequence Download
Gene Name ACO1
Gene ID 512995
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function catalyzes reversible isomerization of citrate to isocitrate; initiates the tricarboxylic acid cycle in the mitochondria
Biochemical Properties two forms of aconitate hydratase (AH) in mammalian tissues differing by their catalytic activity and cellular localization; AH in pig tissues, was found to be associated with two protein fractions of widely different pI (pH 5.43 and 7.50) and stability; as found in rat heart, Specific activity of the purified enzyme samples from the cytoplasmic and mitochondrial fractions of the rat myocardium were 11.2 and 63.6 U/mg; deactivated by iron lability; the absorption spectrum of AH from the rat myocardium has a maximum at 400–440 nm which is specific for Fe-containing proteins; AH from the rat myocardium is active in the wide pH range from 6 to 9; the optimal pH for the cytoplasmic and mitochondrial AH were close to 7.9 and 8.2, respectively
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Bibliography 1. Eanes, R. Z., & Kun, E. (1971). Separation and characterization of aconitate hydratase isoenzymes from pig tissues. Biochimica et Biophysica Acta, 227(1), 204–210. https://doi.org/10.1016/0005-2744(71)90181-1.
2. Medvedeva, L. V, Popova, T. N., Artiukhov, V. G., & Matasova, L. V. (n.d.). [Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes]. Izvestiia Akademii Nauk. Seriia Biologicheskaia, (5), 528–533. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12400375.