Search by BoMiProt ID - Bomi1285


Primary Information

BoMiProt ID Bomi1285
Protein Name Endoplasmin
Organism Bos taurus
Uniprot IDQ95M18
Milk FractionWhey, MFGM, Exosome
Ref Sequence ID NP_777125.1
Aminoacid Length 804
Molecular Weight 92427
FASTA Sequence Download
Gene Name HSP90B1
Gene ID 282646
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function Endoplasmin, or GRP94, is an ER-located stress inducible molecular chaperone implicated in the folding and assembly of many proteins.
PTMs Phosphorylation at Thr/Ser,N6-succinyl at Lysine,N-Linked Glycosylation at Asn,Disulfide bond formation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
Predicted Disorder Regions 288-323,750-804
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs GRP94 is also readily phosphorylated by G-CK at Ser which are different from the sites affected by casein kinase-2 (CK2) in the same protein. A study with peptide substrates would indicate that the G-CK sites in GRP94 conform to the motif S-R/K-E-X (X being different from D and E) which is not recognized by CK2.
Additional Comments Overexpression of cGRP94 in COS-1 cells revealed multiple isoforms including one N-glycosylated species. 
Bibliography 1.Hoter A, Amiri M, Warda M, Naim HY. Molecular cloning, cellular expression and characterization of Arabian camel (Camelus dromedarius) endoplasmin. Int J Biol Macromol. 2018 Oct 1;117:574-585. doi: 10.1016/j.ijbiomac.2018.05.196. Epub 2018 May 27. PMID: 29847784. 2.Brunati AM, Contri A, Muenchbach M, James P, Marin O, Pinna LA. GRP94 (endoplasmin) co-purifies with and is phosphorylated by Golgi apparatus casein kinase. FEBS Lett. 2000 Apr 14;471(2-3):151-5. doi: 10.1016/s0014-5793(00)01378-8. PMID: 10767412.