Search by BoMiProt ID - Bomi116


Primary Information

BoMiProt ID Bomi116
Protein Name Lactotransferrin
Organism Bos taurus
Uniprot IdP24627
Milk FractionWhey
Ref Sequence Id NP_851341.1
Amino Acid Lenth 708
Molecular Weight 78056
Fasta Sequence http://www.uniprot.org/uniprot/P24627.fasta
Gene Name LTF
Gene Id 280846
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondry Information

Presence in other biological fluids/tissue/cells saliva, tears, bile/pncreatic secretions, mucosal secretions of trachea, uterus, ovaries, seminal secretion, plasma, joint fluid
Endogenous/Bioactive peptides - Fragment - Sequence - Effect Lactoferricin - 17–41/42 - FKCRRWQWRMKKLGAPSICURRAF/A - Antimicrobial Ref
Protein Function Potent iron chelator; has antimicrobial, anti-tumor, anti-allergeic, anti-inflammatory activities; promotes innante and adaptive immunity and neutrophil migration; depresses endotoxemia; acts as tumor suppressor in multiple cancers; controls oxidative cell injury; inhibits pathological development during autoimmune disorder; prevents cell apoptosis; used as chemotherapeutic agent
Biochemical Properties Thermostable in acidic environment; optimum pH is 7.5 and temperature is 25°C with 20% α-helix structure; at pH 2-3 structure is partly unfolded with 3% α - helix structure and 54% ß - sheet structure; showed higher thermostability in acidic pH and low ionic strengths; relatively more resistant to heat in acid than in base or neutral solutions ; loses iron binding capacity but retains antimicrobial properties at extreme conditions such as pH 2, 120°C
Significance in milk Highest in colustrum; has biomodulatory properties and effects nutritional value of the milk; used as active ingredients in infant formula, prebiotic drinks and immune supplements; lactoferrin through pepsin digestion produces a more potent anti-microbial peptide, lactoferrisin that reaches the gut
PTMs Glycosylated; 5 N-linked glycans; mixtures of high mannose, complex and hybrid type; 59 different structures have been identified till date; fucosylated and sialylated; presence of unusual motifs including N,N'-diacetyllactosamine as found in bovine milk
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|P24627|TRFL_BOVIN Lactotransferrin OS=Bos taurus OX=9913 GN=LTF PE=1 SV=2
MKLFVPALLS LGALGLCLAA PRKNVRWCTI SQPEWFKCRR WQWRMKKLGA PSITCVRRAF ALECIRAIAE KKADAVTLDG GMVFEAGRDP YKLRPVAAEI YGTKESPQTH YYAVAVVKKG SNFQLDQLQG RKSCHTGLGR SAGWIIPMGI LRPYLSWTES LEPLQGAVAK FFSASCVPCI DRQAYPNLCQ LCKGEGENQC ACSSREPYFG YSGAFKCLQD GAGDVAFVKE TTVFENLPEK ADRDQYELLC LN*252NSRAPVDA FKECHLAQVP SHAVVARSVD GKEDLIWKLL SKAQEKFGKN KSRSFQLFGS PPGQRDLLFK DSALGFLRIP SKVDSALYLG SRYLTTLKNL RETAEEVKAR YTRVVWCAVG PEEQKKCQQW SQQSGQN*387VTC ATASTTDDCI VLVLKGEADA LNLDGGYIYT AGKCGLVPVL AENRKSSKHS SLDCVLRPTE GYLAVAVVKK ANEGLTWNSL KDKKSCHTAV DRTAGWNIPM GLIVN*495QTGSC AFDEFFSQSC APGADPKSRL CALCAGDDQG LDKCVPNSKE KYYGYTGAFR CLAEDVGDVA FVKN*564DTVWEN TNGESTADWA KNLNREDFRL LCLDGTRKPV TEAQSCHLAV APNHAVVSRS DRAAHVKQVL LHQQALFGKN GKNCPDKFCL FKSETKNLLF NDNTECLAKL GGRPTYEEYL GTEYVTAIAN LKKCSTSPLL EACAFLTR
Significance of PTMs Glycosylation changes at multiple points of lactation; glycosylation plays a role in the protein's bioactivity and its digestion profile in the infant gut; source of novel bioactives; stimulation of bone formation; anticancer; sialylation chelates loosed bound Ca2+ and prevent bacterial colonization
PDB ID 2dxr, 2dxy, 2dyx, 2e0s, 2e1s, 2fa7, 2g93, 2h4i, 2hca, 2md1, 2md2, 2md3, 2md4, 2nuv, 2nwj, 2o1l, 2o51, 2ocu, 2p1s, 2px1, 2q8j, 2qje, 2r71, 2r9j, 2zmb, 3cfl, 3ci8, 3crb, 3e9x, 3iaz, 3ib0, 3ib1, 3ib2, 3k0v, 3kj7, 3mjn, 3mjn, 3o97, 3rgy, 3sdf, 3taj, 3tod, 3tus, 3u72, 3u8q, 3ugw, 3uk4, 3usd, 3v5a,3vdf, 4dig, 4dxu, 4fim, 4fjp, 4for, 4g2z, 4g77, 4g8h, 4grk, 4n6p, 4ned, 4oqo, 5cry, 5hbc, 1blf, 1lfc, 1nkx, 1sdx, 1y58, 2alu, 2ays, 2b65, 2doj, 2dp8, 2dqv, 2ds9, 2dsf, 2dvc, 2dwa, 2dwh, 2dwi, 2dwj,
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