Primary Information
BoMiProt ID	Bomi10541
Protein Name	V-type proton ATPase subunit B, kidney isoform/V-ATPase subunit B 1/Endomembrane proton pump 58 kDa subunit/Vacuolar proton pump subunit B 1
Organism	Bos taurus
Uniprot ID	P31407
Milk Fraction	Whey
Ref Sequence ID	NP_788827.1
Aminoacid Length	513
Molecular Weight	56747
FASTA Sequence	Download
Gene Name	ATP6V1B1/ATP6B1
Gene ID	338059
Protein Existence Status	reviewed
Secondary Information
Protein Function	restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion
Biochemical Properties	V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (PubMed:32764564). The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	na
Predicted Disorder Regions	1-21,
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	The amino acid sequence deduced for V-ATPase subunit B of M. sexta is between 98% and 76% identical with that of seven other V-ATPase subunits B and greater than 52% identical with three archaebacterial ATPase subunits B.
Bibliography	1.Novak FJ, Gräf R, Waring RB, Wolfersberger MG, Wieczorek H, Harvey WR. Primary structure of V-ATPase subunit B from Manduca sexta midgut. Biochim Biophys Acta. 1992 Aug 17;1132(1):67-71. doi: 10.1016/0167-4781(92)90053-3. PMID: 1387326. 2.Xia Y, Liu N, Xie X, Bi G, Ba H, Li L, Zhang J, Deng X, Yao Y, Tang Z, Yin B, Wang J, Jiang K, Li Z, Choi Y, Gong F, Cheng X, O'Shea JJ, Chae JJ, Laurence A, Yang XP. The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion. Autophagy. 2019 Jun;15(6):960-975. doi: 10.1080/15548627.2019.1569916. Epub 2019 Jan 29. PMID: 30681394; PMCID: PMC6526827. 3.Wang R, Long T, Hassan A, Wang J, Sun Y, Xie XS, Li X. Cryo-EM structures of intact V-ATPase from bovine brain. Nat Commun. 2020 Aug 6;11(1):3921. doi: 10.1038/s41467-020-17762-9. PMID: 32764564; PMCID: PMC7414150.