Primary Information |
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| BoMiProt ID | Bomi10132 |
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| Protein Name | Tubulin-specific chaperone E/Tubulin-folding cofactor E |
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| Organism | Bos taurus |
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| Uniprot ID | Q32KS0 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001033121.1 |
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| Aminoacid Length | 528 |
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| Molecular Weight | 59327 |
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| FASTA Sequence |
Download |
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| Gene Name | TBCE |
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| Gene ID | 505066 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | enhances microtubule nucleation around chromatin, thereby facilitating mitotic spindle assembly. |
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| Biochemical Properties | There is free exchange of β-tubulin between TBCA and TBCD, and of α-tubulin between TBCB and TBCE, resulting in the formation of TBCD/β and TBCE/α, respectively. The latter two complexes interact, forming a supercomplex (TBCE/α/TBCD/β). Discharge of the native α/β heterodimer occurs via interaction of the supercomplex with TBCC, which results in the triggering of TBC-bound β-tubulin (E-site) GTP hydrolysis. |
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| PTMs | Acetylation and Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q32KS0|TBCE_BOVIN Tubulin-specific chaperone E OS=Bos taurus OX=9913 GN=TBCE PE=2 SV=1
MNSTSTSDVIGRRVEVNGEHATVRFSGLVPPVAGLWLGVEWDNPERGKHDGSHEGTVYFK
CRHPTAGSFIRPHKVNFGVDFLTAIKNRYVLEDEPKEEETEQIVIIGNKPVETIGFDSVI
KQQSQLSKLQDVSLRNCAVNGAGDKGEIAKACPNIRSIDLSKNLLSSWEEVIDIADQLKH
LEVLNLSENKLTSPSSSPSPTGTFPTLKVLVLNRTGVTWAEVLRCASGWPVLEKLYLESN
NIIISERPTDVLQTVKLLDLSSNQLIDENQLFLIAYLPRLEQLILSDIGISSIHFPDAGI
GCKTSMFPSLQYLVLNDNQIAQWSFMNELDKLQSLHALSCTRNPLTEGSKDAQTTRQFII
ARIGQLRTLNKCAIEPEERRGAELDYRKAFGNEWKKAGGHQDPEKNRPNEEFLAAHPRYQ
ALCLKYGAPEDGELKTQQPFLLKNQLLTLKIKYPNQHDQKVIEKQLPDSMTVQKVKGLLS
RLLKVPVSELLLSYES*496PKMPGKEVELENDLQPLRFYSVENGDCLLVRW
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Bibliography | 1.Métivier M, Gallaud E, Thomas A, Pascal A, Gagné JP, Poirier GG, Chrétien D, Gibeaux R, Richard-Parpaillon L, Benaud C, Giet R. Drosophila Tubulin-Specific Chaperone E Recruits Tubulin around Chromatin to Promote Mitotic Spindle Assembly. Curr Biol. 2021 Feb 22;31(4):684-695.e6. doi: 10.1016/j.cub.2020.11.009. Epub 2020 Nov 30. PMID: 33259793. 2.Tian G, Cowan NJ. Tubulin-specific chaperones: components of a molecular machine that assembles the α/β heterodimer. Methods Cell Biol. 2013;115:155-71. doi: 10.1016/B978-0-12-407757-7.00011-6. PMID: 23973072; PMCID: PMC4961357. |
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